(A) Photomicrographs of PPP1R3C siRNA transfected cells in the presence or absence of glucose and with the indicated inhibitors. (B) LDH release into the media from cells in the presence or absence of glucose with or without BafA1. Light bar represents control knockdown cells, whereas dark bars represents PPP1R3C knockdown cells. Measurements were done in triplicate and averaged. Error bars represents S.D. Asterisk indicates P< 0.05 when comparing PPP1R3C knockdown cells in the absence of glucose with and without BafA1. (C) Western blot analysis of the autophagic markers LC3A/B and p62 during glucose deprivation, with or without the indicated inhibitors.