(A) PPP1R3C expression in liver and HepG2 cells transfected with control and knockdown siRNA. Asterisk indicates P< 0.05 when compared with the control knockdown (B) Photomicrographs of siRNA transfected cells in the presence or absence of glucose. (C) LDH release into the media from transfected cells in the presence or absence of glucose, and with or without the indicated inhibitors. Measurements were done in triplicate and averaged. Error bars represents S.D. Asterisk indicates P< 0.05 when compared with the control knockdown cells in the presence of glucose. (D) Western blot analysis showing increased p21 and decreased p34cdc2 levels in the PPP1R3C knockdown cells.