Production of chloroplast PIB-ATPases in Lactococcus membranes
Total membrane proteins (20 μg in lanes 1, 2 and 4–6 or 2 μg in lane 3) were separated by SDS/PAGE. Strep-tag II present at the C-terminus of HMA6 and HMA8 was detected using a Strep-tactin HRP conjugate. The arrows indicate HMA6, HMA8, HMA8-AKT (akt) and HMA8-APA (apa). EV: membrane extract derived from bacteria containing the empty pNZ8148 vector.