(A) Transient transfection of an expression vector for ∆Np73-α into 293FT cells induces the mRNA expression of several proapoptotic genes. (B) Overexpression of ∆Np73-α induces an increment in cells entering apoptosis. 293FT cells transiently transfected as in A were analysed for the induction of apoptosis by anti-Annexin V and propidium iodide immunostaining. A representative experiment is shown. (C) Interference with BIK expression abolishes the apoptosis induced by ∆Np73-α. Cotransfection of shRNA constructs against the indicated proapoptotic genes along with ∆Np73-α reduces the number of cells immunostained for Annexin V.