| Kinase reaction . | |||
|---|---|---|---|
| . | . | Assay type . | |
| Substrate . | Product . | Regeneration . | Standard . |
| Ins1P | - | - | |
| Ins3P | - | ||
| Ins(1,4)P2 | Ins(1,3,4)P3 | ++ | ** |
| Ins(1,3,4)P3 | Ins(1,3,4,5)P4 | + | * |
| Ins(1,4,5)P3 | - | ||
| Ins(1,4,6)P3 | Ins(1,3,4,6)P4 | +++ | *** |
| Ins(3,4,6)P3 | Ins(1,3,4,6)P4 | + | * |
| Ins(3,4,5)P3 | Ins(3,4,5,6)P4 | ** | |
| Ins(4,5,6)P3 | - | ||
| Ins(1,2,4,6)P4 | - | ||
| Ins(2,3,4,6)P4 | - | ||
| Ins(1,3,4,5)P4 | - | ||
| Ins(1,3,5,6)P4 | - | ||
| Ins(1,3,4,6)P4 | - | ||
| Ins(1,4,5,6)P4 | Ins(1,3,4,5,6)P5 | +++ | ** |
| Ins(3,4,5,6)P4 | - | ||
| Ins(1,2,3,4,5)P5 | - | ||
| Ins(1,2,3,5,6)P5 | - | ||
| Ins(1,2,3,4,6)P5 | - | ||
| Ins(1,3,4,5,6)P5 | - | ||
| Ins(1,2,4,5,6)P5 | - | ||
| Ins(2,3,4,5,6)P5 | - | ||
| Ins(1,2,3,4,5,6)P6 | - | ||
| Phosphotransfer to ADP | |||
| Substrate | InsP product | ||
| Ins(1,3,4)P3 | Not verified | ++ | |
| Ins(1,4,6)P3 | Not verified | ++ | |
| Ins(1,3,4,6)P4 | Ins(1,4,6)P3/Ins(3,4,6)P3 | +++ | |
| Ins(1,4,5,6)P4 | Not verified | + | |
| Kinase reaction . | |||
|---|---|---|---|
| . | . | Assay type . | |
| Substrate . | Product . | Regeneration . | Standard . |
| Ins1P | - | - | |
| Ins3P | - | ||
| Ins(1,4)P2 | Ins(1,3,4)P3 | ++ | ** |
| Ins(1,3,4)P3 | Ins(1,3,4,5)P4 | + | * |
| Ins(1,4,5)P3 | - | ||
| Ins(1,4,6)P3 | Ins(1,3,4,6)P4 | +++ | *** |
| Ins(3,4,6)P3 | Ins(1,3,4,6)P4 | + | * |
| Ins(3,4,5)P3 | Ins(3,4,5,6)P4 | ** | |
| Ins(4,5,6)P3 | - | ||
| Ins(1,2,4,6)P4 | - | ||
| Ins(2,3,4,6)P4 | - | ||
| Ins(1,3,4,5)P4 | - | ||
| Ins(1,3,5,6)P4 | - | ||
| Ins(1,3,4,6)P4 | - | ||
| Ins(1,4,5,6)P4 | Ins(1,3,4,5,6)P5 | +++ | ** |
| Ins(3,4,5,6)P4 | - | ||
| Ins(1,2,3,4,5)P5 | - | ||
| Ins(1,2,3,5,6)P5 | - | ||
| Ins(1,2,3,4,6)P5 | - | ||
| Ins(1,3,4,5,6)P5 | - | ||
| Ins(1,2,4,5,6)P5 | - | ||
| Ins(2,3,4,5,6)P5 | - | ||
| Ins(1,2,3,4,5,6)P6 | - | ||
| Phosphotransfer to ADP | |||
| Substrate | InsP product | ||
| Ins(1,3,4)P3 | Not verified | ++ | |
| Ins(1,4,6)P3 | Not verified | ++ | |
| Ins(1,3,4,6)P4 | Ins(1,4,6)P3/Ins(3,4,6)P3 | +++ | |
| Ins(1,4,5,6)P4 | Not verified | + | |
For ATP-regeneration assays, -/+/++/+++ indicates reaction strength within pair of enantiomers or classes (InsP3, InsP4) of compounds; - indicates absence of activity; otherwise, not tested. Without ATP regeneration (standard assay), reaction indicated -/*/**/****. Not verified, InsP2s are poorly resolved on the column used and standards were not used. All reactions which failed to give products were analyzed beside positive controls that yielded products. Structures of substrates/products shown in Supplementary Figure S1.