| Plasmid . | Notes . | Reference . |
|---|---|---|
| pBADcLIC | Empty expression vector. AmpR. | [42] |
| pUC57_noBsaI_XylBco | Cloning vector with codon-optimised XylB. AmpR. | This work; ordered from Biomatik |
| pBADcLIC_XylBco | Expression vector with codon-optimised XylB. AmpR. | This work |
| pBADcLIC_XylBfix | Expression vector with codon-optimised and sequence-corrected XylB. AmpR. | This work |
| pUC57_AcBAD | Cloning vector with codon-optimised AcBAD. AmpR. | This work; ordered from Biomatik |
| pBADcLIC_AcBAD | Expression vector with codon-optimised AcBAD. AmpR. | This work |
| pUC57_BaBAD | Cloning vector with codon-optimised BaBAD. AmpR. | This work; ordered from Biomatik |
| pBADcLIC_BaBAD | Expression vector with codon-optimised BaBAD. AmpR. | This work |
| Strain | ||
| Escherichia coli BW25113 | Δ(araB–D)567 Δ(rhaD–B)568 ΔlacZ4787(::rrnB-3) hsdR514 rph-1 | [59] |
| Plasmid . | Notes . | Reference . |
|---|---|---|
| pBADcLIC | Empty expression vector. AmpR. | [42] |
| pUC57_noBsaI_XylBco | Cloning vector with codon-optimised XylB. AmpR. | This work; ordered from Biomatik |
| pBADcLIC_XylBco | Expression vector with codon-optimised XylB. AmpR. | This work |
| pBADcLIC_XylBfix | Expression vector with codon-optimised and sequence-corrected XylB. AmpR. | This work |
| pUC57_AcBAD | Cloning vector with codon-optimised AcBAD. AmpR. | This work; ordered from Biomatik |
| pBADcLIC_AcBAD | Expression vector with codon-optimised AcBAD. AmpR. | This work |
| pUC57_BaBAD | Cloning vector with codon-optimised BaBAD. AmpR. | This work; ordered from Biomatik |
| pBADcLIC_BaBAD | Expression vector with codon-optimised BaBAD. AmpR. | This work |
| Strain | ||
| Escherichia coli BW25113 | Δ(araB–D)567 Δ(rhaD–B)568 ΔlacZ4787(::rrnB-3) hsdR514 rph-1 | [59] |
For pUC57_noBsaI_xylBco, pUC57_AcBAD and pUC57_BaBAD, the codon-optimised DNA sequences provided by Biomatik can be found in Supplementary Data S1.