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Table 2
Strains and plasmids used in this work
PlasmidNotesReference
pBADcLIC Empty expression vector. AmpR[42] 
pUC57_noBsaI_XylBco Cloning vector with codon-optimised XylB. AmpRThis work; ordered from Biomatik 
pBADcLIC_XylBco Expression vector with codon-optimised XylB. AmpRThis work 
pBADcLIC_XylBfix Expression vector with codon-optimised and sequence-corrected XylB. AmpRThis work 
pUC57_AcBAD Cloning vector with codon-optimised AcBAD. AmpRThis work; ordered from Biomatik 
pBADcLIC_AcBAD Expression vector with codon-optimised AcBAD. AmpRThis work 
pUC57_BaBAD Cloning vector with codon-optimised BaBAD. AmpRThis work; ordered from Biomatik 
pBADcLIC_BaBAD Expression vector with codon-optimised BaBAD. AmpRThis work 
Strain 
Escherichia coli BW25113 Δ(araB–D)567 Δ(rhaD–B)568 ΔlacZ4787(::rrnB-3) hsdR514 rph-1 [59] 
PlasmidNotesReference
pBADcLIC Empty expression vector. AmpR[42] 
pUC57_noBsaI_XylBco Cloning vector with codon-optimised XylB. AmpRThis work; ordered from Biomatik 
pBADcLIC_XylBco Expression vector with codon-optimised XylB. AmpRThis work 
pBADcLIC_XylBfix Expression vector with codon-optimised and sequence-corrected XylB. AmpRThis work 
pUC57_AcBAD Cloning vector with codon-optimised AcBAD. AmpRThis work; ordered from Biomatik 
pBADcLIC_AcBAD Expression vector with codon-optimised AcBAD. AmpRThis work 
pUC57_BaBAD Cloning vector with codon-optimised BaBAD. AmpRThis work; ordered from Biomatik 
pBADcLIC_BaBAD Expression vector with codon-optimised BaBAD. AmpRThis work 
Strain 
Escherichia coli BW25113 Δ(araB–D)567 Δ(rhaD–B)568 ΔlacZ4787(::rrnB-3) hsdR514 rph-1 [59] 

For pUC57_noBsaI_xylBco, pUC57_AcBAD and pUC57_BaBAD, the codon-optimised DNA sequences provided by Biomatik can be found in Supplementary Data S1.

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