Hexokinase II mutations were made in the putative VDAC-binding site located on the N-terminal end
Deletion mutations of 20 amino acids (pI2_Q20del) and 10 amino acids (pI2_F10del) by PCR and the clones analysed for binding to the mitochondrial membrane. Using PredictProtein [14,15], 3 amino acids were predicted to bind protein within the 10 amino acid deletion. Single point mutations at amino acids 4(S4L), 5(H5P) and 8(A8L) were made by PCR and the clones analysed for binding to the mitochondrial membrane. Mutations depicted in red.