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Keywords: CRISPR
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Essays Biochem (2022) 66 (4): 305–318.
Published: 16 September 2022
... outcomes. However, challenges remain due to limited understanding of the molecular mechanisms governing self-renewal and survival of CSCs. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 screening has been increasingly used to identify genetic determinants in cancers. In this primer...
Essays Biochem (2019) 63 (6): 813–825.
Published: 14 November 2019
...Christian Pflueger; Tessa Swain; Ryan Lister DNA methylation is an essential DNA modification that plays a crucial role in genome regulation during differentiation and development, and is disrupted in a range of disease states. The recent development of CRISPR/catalytically dead CRISPR/Cas9 (dCas9...
Essays Biochem (2019) 63 (4): 457–468.
Published: 11 October 2019
... by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . biochemical techniques and resources CRISPR DNA sequencing This article is an updated version of the first Biochemical Society guide to recombinant DNA...
Includes: Supplementary data
Essays Biochem (2019) 63 (2): 227–235.
Published: 11 June 2019
...Chun H. Lau; Ryan Reeves; Edward L. Bolt Prokaryotes can defend themselves against invading mobile genetic elements (MGEs) by acquiring immune memory against them. The memory is a DNA database located at specific chromosomal sites called CRISPRs (clustered regularly interspaced short palindromic...