Using nuclear magnetic resonance (NMR) spectroscopy in the study of metabolism has been immensely popular in medical- and health-related research but has yet to be widely applied to more fundamental biological problems. This review provides some NMR background relevant to metabolism, describes why 1H NMR spectra are complex as well as introducing relevant terminology and definitions. The applications and practical considerations of NMR metabolic profiling and 13C NMR-based flux analyses are discussed together with the elegant ‘enzyme trap’ approach for identifying novel metabolic pathway intermediates. The importance of sample preparation and data analysis are also described and explained with reference to data precision and multivariate analysis to introduce researchers unfamiliar with NMR and metabolism to consider this technique for their research interests. Finally, a brief glance into the future suggests NMR-based metabolism has room to expand in the 21st century through new isotope labels, and NMR technologies and methodologies.
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December 2016
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The metabolome consists of a collection of biomolecules present in cells, tissues, or body fl uids in a certain moment or physiological situation. It refl ects an individual’s genetic background and is modulated by environmental factors such as diet and physical activity. Metabolomics is the comprehensive study of the entire metabolome, made possible due to advances in the areas of mass spectrometry and nuclear magnetic resonance, allowing the systematic investigation of the metabolome in various biological specimens. Metabolic profi les generated through metabolomic approaches can offer insights into pathophysiological mechanisms associated with nutrient dependent dysfunction or disease and targeted therapeutic approaches. Image supplied by Philip Newsholme and Rodrigo Carless - PDF Icon PDF LinkTable of Contents
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Review Article|
December 15 2016
Solution state nuclear magnetic resonance spectroscopy for biological metabolism and pathway intermediate analysis
Gareth L. Nealon;
Gareth L. Nealon
1Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Crawley, Perth, Australia, 6009
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Mark J. Howard
1Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Crawley, Perth, Australia, 6009
2Department of Chemistry and Biochemistry, The University of Western Australia, Crawley, Perth, Australia, 6009
Correspondence: Mark J. Howard (mark.howard@uwa.edu.au)
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Publisher: Portland Press Ltd
Received:
June 17 2016
Revision Received:
October 07 2016
Accepted:
October 10 2016
Online ISSN: 1744-1358
Print ISSN: 0071-1365
© 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society
2016
Essays Biochem (2016) 60 (5): 419–428.
Article history
Received:
June 17 2016
Revision Received:
October 07 2016
Accepted:
October 10 2016
Citation
Philip Newsholme, Gareth L. Nealon, Mark J. Howard; Solution state nuclear magnetic resonance spectroscopy for biological metabolism and pathway intermediate analysis. Essays Biochem 15 December 2016; 60 (5): 419–428. doi: https://doi.org/10.1042/EBC20160044
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