Import of preproteins into chloroplasts is an essential process, requiring two major multisubunit protein complexes that are embedded in the outer and inner chloroplast envelope membrane. Both the translocon of the outer chloroplast membrane (Toc), as well as the translocon of the inner chloroplast membrane (Tic) have been studied intensively with respect to their individual subunit compositions, functions and regulations. Recent advances in crystallography have increased our understanding of the operation of these proteins in terms of their interactions and regulation by conformational switching. Several subdomains of components of the Toc translocon have been studied at the structural level, among them the polypeptide transport-associated (POTRA) domain of the channel protein Toc75 and the GTPase domain of Toc34. In this review, we summarize and discuss the insight that has been gained from these structural analyses. In addition, we present the crystal structure of the Toc64 tetratrico-peptide repeat (TPR) domain in complex with the C-terminal domains of the heat-shock proteins (Hsp) Hsp90 and Hsp70.
Skip Nav Destination
Article navigation
April 2018
Issue Editors
-
Cover Image
Cover Image
Review Article|
February 27 2018
Structural components involved in plastid protein import
Serena Schwenkert;
Serena Schwenkert
1Munich Center for Integrated Protein Science at the Ludwig-Maximilians-Universität München, Department of Biology I, Botany, Großhaderner Straße 2–4, Martinsried D-82152, Germany
Search for other works by this author on:
Sophie Dittmer;
Sophie Dittmer
1Munich Center for Integrated Protein Science at the Ludwig-Maximilians-Universität München, Department of Biology I, Botany, Großhaderner Straße 2–4, Martinsried D-82152, Germany
Search for other works by this author on:
Jürgen Soll
1Munich Center for Integrated Protein Science at the Ludwig-Maximilians-Universität München, Department of Biology I, Botany, Großhaderner Straße 2–4, Martinsried D-82152, Germany
Correspondence: Jürgen Soll (soll@lmu.de)
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
September 22 2017
Revision Received:
January 24 2018
Accepted:
January 29 2018
Online ISSN: 1744-1358
Print ISSN: 0071-1365
© 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2018
Essays Biochem (2018) 62 (1): 65–75.
Article history
Received:
September 22 2017
Revision Received:
January 24 2018
Accepted:
January 29 2018
Citation
Steven Gutteridge, Serena Schwenkert, Sophie Dittmer, Jürgen Soll; Structural components involved in plastid protein import. Essays Biochem 13 April 2018; 62 (1): 65–75. doi: https://doi.org/10.1042/EBC20170093
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.