NMR (nuclear magnetic resonance) investigation through the exploitation of paramagnetic effects is passing from an approach limited to few specialists in the field to a generally applicable method that must be considered, especially for the characterization of systems hardly affordable with other techniques. This is mostly due to the fact that paramagnetic data are long range in nature, thus providing information for the structural and dynamic characterization of complex biomolecular architectures in their native environment. On the other hand, this information usually needs to be complemented by data from other sources. Integration of paramagnetic NMR with other techniques, and the development of protocols for a joint analysis of all available data, is fundamental for achieving a comprehensive characterization of complex biological systems. We describe here a few examples of the new possibilities offered by paramagnetic data used in integrated structural approaches.
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Cover Image
Cover Image
Cryo-STX projection image showing a parasitophorous vacuole (yellow) within a human fibroblast cell, containing four Toxoplasma gondii parasites (membranes in cyan, nuclei in red and rhoptries in green). In this issue of Emerging Topics in Life Sciences, Harkiolaki et al. describe the use of Cryo-soft X-ray tomography to explore the ultrastructure of whole cells. Image kindly provided by Professor Helen Saibil (Birkbeck College, London, U.K.). For further details, see pages 81–92.
Paramagnetic NMR as a new tool in structural biology
Marcellus Ubbink, Anastassis Perrakis, Enrico Ravera, Azzurra Carlon, Marco Fragai, Giacomo Parigi, Claudio Luchinat; Paramagnetic NMR as a new tool in structural biology. Emerg Top Life Sci 20 April 2018; 2 (1): 19–28. doi: https://doi.org/10.1042/ETLS20170084
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