Genome-editing technologies based on the CRISPR (clustered regularly interspaced short palindromic repeat) system have been widely used in plants to investigate gene function and improve crop traits. The recently developed DNA-free delivery methods and precise base-editing systems provide new opportunities for plant genome engineering. In this review, we describe the novel DNA-free genome-editing methods in plants. These methods reduce off-target effects and may alleviate regulatory concern about genetically modified plants. We also review applications of base-editing systems, which are highly effective in generating point mutations and are of great value for introducing agronomically valuable traits. Future perspectives for DNA-free editing and base editing are also discussed.
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November 2017
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Cover Image
Cover Image
Efficient regeneration from wheat immature embryos. Successful gene editing in crops depends on the regeneration capacity of the selected target tissue. Image kindly provided by Wendy Harwood (John Innes Centre, Norwich).
Review Article|
November 10 2017
Recent advances in DNA-free editing and precise base editing in plants
Yi Zhang;
Yi Zhang
1Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan, China
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Caixia Gao
2State Key Laboratory of Plant Cell and Chromosome Engineering, and Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
Correspondence: Caixia Gao (cxgao@genetics.ac.cn)
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Publisher: Portland Press Ltd
Received:
July 10 2017
Revision Received:
September 13 2017
Accepted:
September 19 2017
Online ISSN: 2397-8562
Print ISSN: 2397-8554
© 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society and the Royal Society of Biology
2017
Emerg Top Life Sci (2017) 1 (2): 161–168.
Article history
Received:
July 10 2017
Revision Received:
September 13 2017
Accepted:
September 19 2017
Citation
Wendy Harwood, Yi Zhang, Caixia Gao; Recent advances in DNA-free editing and precise base editing in plants. Emerg Top Life Sci 10 November 2017; 1 (2): 161–168. doi: https://doi.org/10.1042/ETLS20170021
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