... and myofibrillar protein) were obtained during the fractionation procedure. Key words cytochrome c oxidase, fatiguability, flooding dose, muscle wasting, subcellular fractionation, zymosan. Abbreviations: FSR, fractional synthesis rate; SA, specific activity. Correspondem Dr 0. Rooyackers. Endocrine Research Unit...

... The Biochemical Society and the Medical Research Society 1993 alcohol free radical iron liver mitochondria peroxisome electron paramagnetic resonance spectroscopy subcellular fractionation Clinical Science (1993) 84, 339-348 (Printed in Great Britain) 339 Electron paramagnetic resonance...

... with an undefined form of primary hyperoxaluria (possibly oxalate hyperabsorption) had normal AGT levels. The livers of a very mild PH1-type variant and a PH1 heterozygote had intermediate levels of AGT activity. 4. Subcellular fractionation of four PH1 livers by sucrose gradient isopycnic centrifugation...

...T. J. Peters; W. Jenkins; V. Dubowitz 1. An homogenate of liver biopsy taken from a patient with Pompe's disease (type II glycogen-storage disease) was analysed by enzyme microassay and subcellular fractionation by sucrose-density-gradient centrifugation. 2. A strikingly low activity of lysosomal...

... 8 1978 15 12 1978 © 1979 The Biochemical Society and the Medical Research Society 1979 basal—lateral membrane brush borders cytosol endoplasmic reticulum enzymes giardiasis lysosomes mitochondria subcellular fractionation tropical malabsorption Clinical Science (1979...

...J. M. P. Hopkins; T. J. Peters 1. 59 Fe-labelled ferric chloride was introduced into tied loops of guinea-pig jejunum. 2. After 5–30 min the loop was removed, the enterocytes were isolated, homogenized and subjected to analytical subcellular fractionation. 3. Uptake of 59 Fe was extremely rapid...

... organelles were determined in the isolated cell homogenates and compared with whole liver homogenates. 3. The cells were disrupted and the extracts subjected to analytical subcellular fractionation by sucrose-density-gradient centrifugation. Lysosomal integrity was determined by assaying latent β- N...

... The Biochemical Society and the Medical Research Society 1978 brush border enterocyte ileum intestine jejunum steroid subcellular fractionation Clinical Science and Molecular Medicine (1 978) 55,435-443 The effects of prednisolone on the rat enterocyte at a subcellular level R . M. B A T T , G. W E...

... Society 1978 alcoholic liver disease enzymes ethanol lysosomes microsomes mitochondria subcellular fractionation plasma membrane Clinical Science and Molecular Medicine (1978) 55, 383-389 Changes in hepatic enzymes and organelles in alcoholic liver disease C A R O L A. S E Y M O U R * A N...

...T. J. Peters; P. E. Jones; G. Wells 1. Portions of jejunal biopsy specimens from patients with coeliac disease were homogenized in isotonic sucrose solution and subjected to analytical subcellular fractionation by sucrose-density-gradient centrifugation. 2. The activities and distribution of marker...

...T.J. Peters; P.E. Jones; W.J. Jenkins; Gill Wells 1. Jejunal biopsies from five patients with non-responsive coeliac disease have been subjected to analytical subcellular fractionation and enzymic microassay in order to compare the organelle pathology of this group with untreated but gluten...

... fragility. 12 10 1977 13 3 1978 © 1978 The Biochemical Society and the Medical Research Society 1978 alkaline phosphatase brush border enterocyte ileum intestine jejunum subcellular fractionation Clinical Science and Molecular Medicine (1978) 55,157-165 Analytical subcellular...

...R. W. Lobley; Ruth Franks; R. Holmes 1. Specimens of human duodenal mucosa were obtained at duodenotomy. Superficial mucosal scrapings were homogenized in isotonic sucrose solution and fractionated by differential centrifugation. The distribution of organelles among the subcellular fractions...

... mitochondria myocardium sarcolemma subcellular fractionation Clinical Science and Molecular Medicine (1917) 53, 63-74. Analytical subcellular fractionation of guinea-pig myocardium F. J. BLOOMFIELD, G. WELLS, E . WELMAN AND T. J . PETERS Department of Medicine, Royal Postgraduate Medical School, London...

...A. W. Segal; T. J. Peters 1. Granulocytes were isolated from 40–400 ml of human blood, disrupted and subjected to analytical subcellular fractionation by centrifugation on sucrose density gradients. 2. The principal subcellular organelles, characterized by their respective marker enzymes, were...

...Carol A. Seymour; G. Neale; T. J. Peters 1. Clinical, morphological and biochemical data, including data obtained from the application of subcellular fractionation techniques to liver biopsy specimens, are presented for two patients with the Dubin-Johnson-Sprinz (DJS) syndrome. 2. Subcellular...

... membrane subcellular fractionation jejunum Clinical Science and Molecular Medicine (1976) 51, 557-574. Analytical subcellular fractionation of jejunal biopsy specimens : methodology and characterization of the organelles in normal tissue T. J. PETERS Department of Medicine, Royal Postgraduate Medical...

... bleomycin entrapped in liposomes (2.0 mCi). Scans were recorded on a whole- body linear scanner and a large-field gamma camera. Subcellular fractionation of liver biopsies Portions of closed needle liver biopsies (1.5 mg of protein, 15 640 c.p.m.) were fractionated by sucrose- density-gradient...

... and Molecular Medicine (1976) 50, 355-366. Analytical subcellular fractionation studies on rat liver and on isolated jejunal enterocytes with special reference to the separation of lysosomes, peroxisomes and mitochondria T. J . PETERS AND H. SHIO Royal Postgraduate Medical School, London, and The Rockefeller...

... The Biochemical Society and the Medical Research Society 1975 granulocytes leucocytes lysosomes microsomes mitochondria plasma membranes subcellular fractionation vitamin B 12 vitamin B 12 -binding proteins Clinical Science and Molecular Medicine (1975) 49, 171-1 82 Analytical subcellular...