... endotoxin tolerance in endothelial cells. Most importantly, MPLA, a vaccine adjuvant with a wide therapeutic window, induced tolerance to LPS in endothelial cells. RNA was isolated from cultured cells via GenElute Mammalian Total RNA Miniprep Kit (Sigma) following the manufacturer's instructions at 6 h...

... at non-weight-bearing joints which cannot be explained by biomechanical factors. endotoxaemia endotoxin lipopolysaccharide obesity osteoarthritis CRP is another pro-inflammatory factor positively correlated with BMI in otherwise healthy individuals, and its elevation in obesity may...

...Rokhsareh F. Vesali; Norbert Cibicek; Towe Jakobsson; Maria Klaude; Jan Wernerman; Olav Rooyackers The human endotoxin model has been used to study the early phase of sepsis. The aim of the present study was to assess leg muscle protein kinetics after an endotoxin challenge given to healthy human...

... endotoxin [LPS (lipopolysaccharide), 20 units/kg of body mass; n =8] or placebo ( n =9) in healthy males. L -Arginine and dimethylarginines were quantified after solid-phase extraction by reversed-phase HPLC. Body temperature, heart rate and leucocyte count increased after LPS administration ( P <0.01...

... expression in normal mice and endotoxin [lipopolysaccharide (LPS)]-tolerant mice. The model of LPS tolerance was used because it results in patterns of cytokine expression similar to those commonly observed following severe trauma or sepsis. Glucan treatment potentiated LPS-induced IFN-γ expression...

..., TX 77555–0876, U.S.A. (e-mail theming@utmb.edu ). 20 4 2001 18 5 2001 The Biochemical Society and the Medical Research Society © 2001 2001 acidbase cytokine endotoxin leucocyte tumour necrosis factor- 267Clinical Science (2001) 101, 267 274 (Printed in Great Britain...

... other than microvascular recruitment. NO appears to increase microvascular permeability in endotoxaemia, an effect that may be attenuated by L -NAME, particularly in the lung. capillary density endotoxin microvascular permeability sepsis Correspondence : Professor Timothy W. Evans (e...

...Sun-Sang WANG; Fa-Yauh LEE; Che-Chang CHAN; Rei-Hwa LU; Yee CHAO; Han-Chieh LIN; Shwu-Ling WU; Yang-Te TSAI; Shou-Dong LEE To delineate the clinical roles of plasma cytokine or endotoxin levels in the natural course of infection in patients with decompensated cirrhosis, 66 cirrhotic patients were...

... and regional blood flows. Endotoxin, tumour necrosis factor-alpha (TNF-α) and nitric oxide (NO) have been reported to be involved in the pathogenesis of hyperdynamic circulation, but the interactions between endotoxin, TNF-α and NO in cirrhotic rats with ascites have never been specifically addressed. 2...

... of the inflammatory response to exercise were measured: polymorphonuclear myeloperoxidase (MPO), anaphylatoxin C5a (C5a), tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Plasma endotoxin was measured by a sensitive and rapid chromogenic Limulus assay. All inflammatory markers were significantly increased...

... an inflammatory challenge by intraperitoneal injection of tumour necrosis factor-α or endotoxin (lipopolysaccharide). 3. Diets containing 200 g of casein and 8 g of l-cysteine/kg (normal-protein diet), or 80 g of casein and 8 g of l-cysteine, or isonitrogenous amounts of l-methionine or l-alanine (low-protein...

... contrast to the deleterious consequences of nitro-l-arginine methyl ester, a non-selective nitric oxide synthase inhibitor, in similar conditions. 7 9 1995 8 1 1996 16 1 1996 © 1996 The Biochemical Society and the Medical Research Society 1996 l-canavanine endotoxin mouse...

...H. T. Besler; R. F. Grimble 1. n -3 polyunsaturated fatty acids decrease responses to cytokines and inflammatory agents. The present study examines how different intakes of n -6 and n -9 fatty acids influence the metabolic response to endotoxin in Wistar rats. 2. Weanling male rats were, for 4...

... behaviour of an intravenously injected bolus of recombinant human tumour necrosis factor-α (50 μg/m 2 ) in another group of six normal subjects. We then calculated the total amount of tumour necrosis factor-α produced after intravenous injection of endotoxin (2 ng/kg) as the product of the systemic...

...H. Terence Cook; Alison J. Bune; Albertine S. Jansen; G. Michael Taylor; Rashpal K. Loi; Victoria Cattell 1. Endotoxin induces a shock-like syndrome with increased nitric oxide synthesis. To clarify the cellular source of NO in endotoxic shock we used immunohistochemistry and in situ hybridization...

...Tom van der Poll; Hans P. Sauerwein © 1993 The Biochemical Society and the Medical Research Society 1993 cytokines endotoxin fat glucose metabolism sepsis stress hormones tumor necrosis factor-α Clinical Science (1993) 84, 247-256 (Printed in Great Britain) Editorial Review...

... failure syndrome and death within 42 h of a continuous intravenous infusion of Escherichia coli endotoxin (40 ng min −1 kg −1 ). 2. Plasma and pulmonary lymph endothelin-like immunoreactivity were measured by r.i.a. using a specific antiserum raised in rabbits against porcine endothelin-1. Endothelin-like...

... endotoxin Escherichia coli Gram-negative bacteraemia lipopolysaccharide tumour necrosis factor-α Clinical Science (1 990) 79, 6 19-623 619 Endothelin immunoreactivity in mice with Gram-negative bacteraemia: relationship to tumour necrosis factor- a KAZUHIRO TAKAHASHI, AYONA SILVA*, JONATHAN COHEN...

... of glycolysis to insulin. 4. Endotoxaemia decreased the rates of glycogen synthesis at all concentrations of insulin both at 24 h (∼ 39%) and 48 h (∼ 23%). 5. The increase in the rate of glycolysis was related in a dose-dependent manner to the amount of endotoxin given to the animals. 6. Endotoxaemia decreased...

... with the polymorphonuclear leucocytes in suspension. Bacterial endotoxin in concentrations above 6 μg/ml significantly increased fibronectin digestion by neutrophils, but leukotriene B 4 , interferon-μ and interleukin-1α had no significant effects. 3. Dexamethasone inhibited fibronectin digestion by neutrophils in a dose...

...S. A. Ash; G. E. Griffin 1. Intravenous infusion of endotoxin into rats over 18 h caused a reduction in food intake to 20% of normal levels, weight loss, hypoalbuminaemia and a fall in rates of protein synthesis in vivo in heart and skeletal muscle. 2. Measurements of protein turnover in vitro...

... toxic substances present in the serum of patients with FHF. 3. Chenodeoxycholic acid, p -hydroxyphenyllactic acid, p -hydroxyphenylpyruvic acid and endotoxin caused 30–45% inhibition of partially purified rat brain Na + ,K + -ATPase at concentrations known to occur in the serum of patients with FHF...

...J. P. Nolan; R. C. Venuto; Gwendolyn S. Goldmann 1. A relationship between bacterial endotoxin absorbed from the gut and acute renal failure has been postulated. Experiments employing either the endotoxin-tolerant state or the enhancement of endotoxin injury were undertaken to test...