... (alkaline phosphatase, γ-glutamyl transferase) localized at least in part to the brush border. Other enzymes (leucyl-2-naphthylamidase, α-glucosidase) were unaffected. 3. Density gradient centrifugation confirmed the selective decrease in the brush-border activity but the equilibrium density...

... was monitored by assay of suitable marker enzymes. 2. Enterokinase was recovered predominantly in the nuclear + brush-border fraction and 80% of the total activity was found to be particulate; approximately 20% of the enzyme was present in the soluble fraction, compared with 1% of the brush-border markers...

... densities in sucrose density gradients: brush borders (1·21), peroxisomes (1·18), mitochondria (1·16), endoplasmic reticulum (1·16), basal—lateral membranes (1·12). At least three distinct populations of lysosomes with different modal densities and enzyme content were demonstrated. 5. This analytical...

...: brush borders, galactose absorption, lysosomes, rat enterocyte, triparanol. Correspondence: Dr T. J. Peters, Department of Medicine, Royal Postgraduate Medical School, Du Cane Road, London W12 OHS. Introduction Triparanol, a drug which inhibits the synthesis of cholesterol, may cause malabsorption...

... and enterocytes were compared. Key words: brush borders, cytosol, enterocytes, intestine, liver, lysosomes, microsomes, mito- chondria, peroxisomes, subcellular fractionation. Introduction There have been several previous attempts to perform subcellular fractionation of cells of the small intestine but most...

...T. J. Peters; J. R. Heath; M. H. Wansbrough-Jones; W. F. Doe 1. Reduced activities of four enzymes from brush borders were found in intestinal biopsies from patients with untreated coeliac disease. The activities returned towards control values after treatment by gluten withdrawal. Parallel changes...

...T. J. Peters 1. The relative rates of hydrolysis of a series of glycine homopeptides by guinea-pig small intestinal mucosa and by isolated brush borders have been studied. Oligopeptides up to and including hexaglycine were hydrolysed. No activity was detected against a series of homopolypeptides...