Coronary artery ectasia (CAE) is a disease characterized by abnormally dilated coronary arteries. The mechanism of CAE remains unclear, and its treatment is limited. Previous studies have shown that risk factors for CAE were related to changes in DNA methylation. However, no systematic investigation of methylation profiles has been performed. Therefore, we compared methylation profiles between 12 CAE patients and 12 propensity-matched individuals with normal coronary arteries using microarrays. Wilcoxon's rank sum tests revealed 89 genes with significantly different methylation levels (P<0.05 and Δβ > |0.1|). Functional characterization using the DAVID database and gene set enrichment analysis indicated that these genes were involved in immune and inflammatory responses. Of these genes 6 were validated in 29 CAE patients and 87 matched individuals with CAE, using pyro-sequencing. TLR6 and NOTCH4 showed significant differences in methylation between the two groups, and lower protein levels of toll-like receptor 6 (TLR6) were detected in CAE patients. In conclusion, this genome-wide analysis of methylation profiles in CAE patients showed that significant changes in both methylation and expression of TLR6 deserve further study to elucidate their roles in CAE.
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Research Article|
March 17 2017
Genome-wide methylation profiles in coronary artery ectasia
Tzu-Pin Lu;
Tzu-Pin Lu
1Institute of Epidemiology and Preventive Medicine, Department of Public Health, National Taiwan University, Taiwan
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Nai-Chen Chuang;
Nai-Chen Chuang
1Institute of Epidemiology and Preventive Medicine, Department of Public Health, National Taiwan University, Taiwan
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Chin-Yu Cheng;
Chin-Yu Cheng
2Department of Laboratory Medicine, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taiwan
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Cheng-An Hsu;
Cheng-An Hsu
2Department of Laboratory Medicine, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taiwan
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Yi-Chih Wang;
Yi-Chih Wang
3Cardiovascular Center and Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital, Taiwan
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Yen-Hong Lin;
Yen-Hong Lin
3Cardiovascular Center and Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital, Taiwan
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Jen-Kuang Lee;
Jen-Kuang Lee
3Cardiovascular Center and Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital, Taiwan
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Cho-Kai Wu;
Cho-Kai Wu
3Cardiovascular Center and Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital, Taiwan
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Juey-Jen Hwang;
Juey-Jen Hwang
3Cardiovascular Center and Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital, Taiwan
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Lian-Yu Lin;
Lian-Yu Lin
3Cardiovascular Center and Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital, Taiwan
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Shih-Fan Sherri Yeh;
Shih-Fan Sherri Yeh
4Department of Medical Education, National Taiwan University Hospital and National Taiwan University College of Medicine, Taiwan
5Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taiwan
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Kuo-Liang Chien;
Kuo-Liang Chien
1Institute of Epidemiology and Preventive Medicine, Department of Public Health, National Taiwan University, Taiwan
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Jyh-Ming Jimmy Juang
3Cardiovascular Center and Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital, Taiwan
Correspondence: Jyh-Ming Jimmy Juang (p91421019@ntu.edu.tw)
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Publisher: Portland Press Ltd
Received:
October 29 2016
Revision Received:
January 13 2017
Accepted:
January 30 2017
Accepted Manuscript online:
January 31 2017
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2017 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society
2017
Clin Sci (Lond) (2017) 131 (7): 583–594.
Article history
Received:
October 29 2016
Revision Received:
January 13 2017
Accepted:
January 30 2017
Accepted Manuscript online:
January 31 2017
Citation
Tzu-Pin Lu, Nai-Chen Chuang, Chin-Yu Cheng, Cheng-An Hsu, Yi-Chih Wang, Yen-Hong Lin, Jen-Kuang Lee, Cho-Kai Wu, Juey-Jen Hwang, Lian-Yu Lin, Shih-Fan Sherri Yeh, Kuo-Liang Chien, Jyh-Ming Jimmy Juang; Genome-wide methylation profiles in coronary artery ectasia. Clin Sci (Lond) 1 April 2017; 131 (7): 583–594. doi: https://doi.org/10.1042/CS20160821
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