CD (coeliac disease) is a frequent autoimmune disorder of the small bowel, which is characterized by an immunological reaction against gluten and transglutaminase in genetically predisposed subjects. However, the molecular determinants underpinning CD pathogenesis are yet to be fully elucidated and little data are available about the involvement of miRNAs (microRNAs) in CD. In the present study, the duodenal mucosa miRNA expression was profiled in adult untreated CD presenting with a classic phenotype or iron-deficiency anaemia, treated patients with or without duodenal normalization, and non-CD subjects as controls. Deregulation of seven miRNAs (miR-31-5p, miR-192-3p, miR-194-5p, miR-551a, miR-551b-5p, miR-638 and miR-1290) was determined in a larger series of CD patients with different clinical phenotypes compared with non-CD subjects. These seven microRNAs were then analysed in duodenal fibroblasts obtained from CD patients and incubated with gliadin peptides (13- and 33-mer). The miRNA cluster miR-192/194, involved in matrix remodelling, was deregulated in CD according to the different clinical presentations, and miR-192-3p levels were modulated by gliadin peptides in vitro. In conclusion, the analysis of miRNAs deserves further consideration for its potential use in the treatment and management of CD.
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Research Article|
November 15 2013
microRNA profiles in coeliac patients distinguish different clinical phenotypes and are modulated by gliadin peptides in primary duodenal fibroblasts
Valentina Vaira;
Valentina Vaira
*Division of Pathology, Fondazione IRCCS Ca’ Granda–Ospedale Maggiore Policlinico, Milan, Italy
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Leda Roncoroni;
Leda Roncoroni
†Center for the Prevention and Diagnosis of Celiac Disease and Gastroenterology 2, Fondazione IRCCS Ca’ Granda–Ospedale Maggiore Policlinico, University of Milan Medical School, Milan, Italy
‡Department of Biomedical, Surgical and Dental Sciences, University of Milan Medical School, Milan, Italy
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Donatella Barisani;
Donatella Barisani
§Department of Experimental Medicine, University of Milano-Bicocca, Monza, Italy
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Gabriella Gaudioso;
Gabriella Gaudioso
*Division of Pathology, Fondazione IRCCS Ca’ Granda–Ospedale Maggiore Policlinico, Milan, Italy
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Silvano Bosari;
Silvano Bosari
*Division of Pathology, Fondazione IRCCS Ca’ Granda–Ospedale Maggiore Policlinico, Milan, Italy
∥Department of Pathophysiology and Organ Transplants, University of Milan Medical School, Milan, Italy
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Gaetano Bulfamante;
Gaetano Bulfamante
¶Department of Health Sciences and Division of Pathology, University of Milan Medical School and A.O. S. Paolo, Milan, Italy
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Luisa Doneda;
Luisa Doneda
‡Department of Biomedical, Surgical and Dental Sciences, University of Milan Medical School, Milan, Italy
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Dario Conte;
Dario Conte
†Center for the Prevention and Diagnosis of Celiac Disease and Gastroenterology 2, Fondazione IRCCS Ca’ Granda–Ospedale Maggiore Policlinico, University of Milan Medical School, Milan, Italy
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Carolina Tomba;
Carolina Tomba
†Center for the Prevention and Diagnosis of Celiac Disease and Gastroenterology 2, Fondazione IRCCS Ca’ Granda–Ospedale Maggiore Policlinico, University of Milan Medical School, Milan, Italy
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Maria Teresa Bardella;
Maria Teresa Bardella
†Center for the Prevention and Diagnosis of Celiac Disease and Gastroenterology 2, Fondazione IRCCS Ca’ Granda–Ospedale Maggiore Policlinico, University of Milan Medical School, Milan, Italy
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Stefano Ferrero;
Stefano Ferrero
*Division of Pathology, Fondazione IRCCS Ca’ Granda–Ospedale Maggiore Policlinico, Milan, Italy
‡Department of Biomedical, Surgical and Dental Sciences, University of Milan Medical School, Milan, Italy
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Martina Locatelli;
Martina Locatelli
†Center for the Prevention and Diagnosis of Celiac Disease and Gastroenterology 2, Fondazione IRCCS Ca’ Granda–Ospedale Maggiore Policlinico, University of Milan Medical School, Milan, Italy
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Luca Elli
†Center for the Prevention and Diagnosis of Celiac Disease and Gastroenterology 2, Fondazione IRCCS Ca’ Granda–Ospedale Maggiore Policlinico, University of Milan Medical School, Milan, Italy
Correspondence: Dr Luca Elli (email lucelli@yahoo.com).
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Publisher: Portland Press Ltd
Received:
May 21 2013
Revision Received:
September 06 2013
Accepted:
September 24 2013
Accepted Manuscript online:
September 24 2013
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2014 Biochemical Society
2014
Clin Sci (Lond) (2014) 126 (6): 417–423.
Article history
Received:
May 21 2013
Revision Received:
September 06 2013
Accepted:
September 24 2013
Accepted Manuscript online:
September 24 2013
Citation
Valentina Vaira, Leda Roncoroni, Donatella Barisani, Gabriella Gaudioso, Silvano Bosari, Gaetano Bulfamante, Luisa Doneda, Dario Conte, Carolina Tomba, Maria Teresa Bardella, Stefano Ferrero, Martina Locatelli, Luca Elli; microRNA profiles in coeliac patients distinguish different clinical phenotypes and are modulated by gliadin peptides in primary duodenal fibroblasts. Clin Sci (Lond) 1 March 2014; 126 (6): 417–423. doi: https://doi.org/10.1042/CS20130248
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