Dysregulated VLDL-TAG (very-low-density lipoprotein triacylglycerol) metabolism in obesity may account for hypertriacylglycerolaemia and increased cardiovascular disease. ω-3 FAEEs (omega-3 fatty acid ethyl esters) decrease plasma TAG and VLDL concentrations, but the mechanisms are not fully understood. In the present study, we carried out a 6-week randomized, placebo-controlled study to examine the effect of high-dose ω-3 FAEE supplementation (3.2 g/day) on the metabolism of VLDL-TAG in obese men using intravenous administration of d5-glycerol. We also explored the relationship of VLDL-TAG kinetics with the metabolism of VLDL-apo (apolipoprotein) B-100 and HDL (high-density lipoprotein)-apoA-I. VLDL-TAG isotopic enrichment was measured using gas chromatography-mass spectrometry. Kinetic parameters were derived using a multicompartmental model. Compared with placebo, ω-3 FAEE supplementation significantly lowered plasma concentrations of total (−14%, P<0.05) and VLDL-TAG (−32%, P<0.05), as well as hepatic secretion of VLDL-TAG (−32%, P<0.03). The FCR (fractional catabolic rate) of VLDL-TAG was not altered by ω-3 FAEEs. There was a significant association between the change in secretion rates of VLDL-TAG and VLDL-apoB-100 (r=0.706, P<0.05). However, the change in VLDL-TAG secretion rate was not associated with change in HDL-apoA-I FCR (r=0.139, P>0.05). Our results suggest that the TAG-lowering effect of ω-3 FAEEs is associated with the decreased VLDL-TAG secretion rate and hence lower plasma VLDL-TAG concentration in obesity. The changes in VLDL-TAG and apoB-100 kinetics are closely coupled.
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Research Article|
March 13 2013
Omega-3 fatty acid ethyl ester supplementation decreases very-low-density lipoprotein triacylglycerol secretion in obese men
Annette T. Y. Wong;
Annette T. Y. Wong
*School of Medicine and Pharmacology, University of Western Australia, Perth, WA, Australia
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Dick C. Chan;
Dick C. Chan
*School of Medicine and Pharmacology, University of Western Australia, Perth, WA, Australia
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Esther M. M. Ooi;
Esther M. M. Ooi
*School of Medicine and Pharmacology, University of Western Australia, Perth, WA, Australia
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Theodore W. K. Ng;
Theodore W. K. Ng
*School of Medicine and Pharmacology, University of Western Australia, Perth, WA, Australia
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Gerald F. Watts;
Gerald F. Watts
*School of Medicine and Pharmacology, University of Western Australia, Perth, WA, Australia
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P. Hugh R. Barrett
*School of Medicine and Pharmacology, University of Western Australia, Perth, WA, Australia
†Faculty of Engineering, Computing and Mathematics, University of Western Australia, Perth, WA, Australia
Correspondence: Professor Hugh Barrett (email hugh.barrett@uwa.edu.au).
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Publisher: Portland Press Ltd
Received:
October 29 2012
Revision Received:
January 17 2013
Accepted:
January 28 2013
Accepted Manuscript online:
January 28 2013
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2013 Biochemical Society
2013
Clin Sci (Lond) (2013) 125 (1): 45–51.
Article history
Received:
October 29 2012
Revision Received:
January 17 2013
Accepted:
January 28 2013
Accepted Manuscript online:
January 28 2013
Citation
Annette T. Y. Wong, Dick C. Chan, Esther M. M. Ooi, Theodore W. K. Ng, Gerald F. Watts, P. Hugh R. Barrett; Omega-3 fatty acid ethyl ester supplementation decreases very-low-density lipoprotein triacylglycerol secretion in obese men. Clin Sci (Lond) 1 July 2013; 125 (1): 45–51. doi: https://doi.org/10.1042/CS20120587
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