The aim of the present study was to investigate whether CBSCs [(umbilical) cord blood stem cells] can be a new source of DCs (dendritic cells), which can generate more potent antigen-specific immune responses and anti-tumour effects. CBSCs and PBMCs (peripheral blood mononuclear cells) were collected, cultured and differentiated into DCs. Surface markers, secreting cytokines, antigen-presentation activity, antigen-specific cell-mediated immunity and cytotoxic killing effects induced by these two DC origins were evaluated and compared. CBSCs were expanded ~17-fold by ex vivo culture. The expression of surface markers in CBSC-derived DCs were higher than those in PBMC-derived DCs treated with LPS (lipopolysaccharide). The CBSC-derived DCs mainly secreted IL (interleukin)-6, IL-10 and TNF (tumour necrosis factor)-α, whereas PBMC-derived DCs mainly secreted IL-5 and IFN (interferon)-γ. The CBSC-derived DCs had better antigen-presentation abilities when stimulated with LPS or TNF-α, induced higher numbers of IFN-γ-secreting antigen-specific CD8+ T-cells, as assessed using an ELISpot (enzyme-linked immunosorbent spot) assay, and stimulated more potent antigen-specific CTL (cytotoxic T-cell) activities (P<0.01, one-way ANOVA). CBSC-derived DCs had quicker and greater ERK (extracellular-signal-regulated kinase) and Akt phosphorylation, and weaker p38 phosphorylation, than PBMC-derived DCs when stimulated with LPS. In conclusion, CBSC-derived DCs have the ability to induce stronger antigen-specific immunity and more potent anti-tumour effects and therefore could be a good source of DCs for use in DC-based cancer vaccines and immunotherapy.
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Research Article|
May 29 2012
Cord blood stem-cell-derived dendritic cells generate potent antigen-specific immune responses and anti-tumour effects
Ming-Cheng Chang;
Ming-Cheng Chang
*Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Taiwan
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Chien-Nan Lee;
Chien-Nan Lee
*Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Taiwan
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Yu-Li Chen;
Yu-Li Chen
*Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Taiwan
†Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan
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Ying-Cheng Chiang;
Ying-Cheng Chiang
*Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Taiwan
‡Department of Obstetrics and Gynecology, National Taiwan University Yun-Lin Branch, Yun-Lin County, Taiwan
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Wei-Zen Sun;
Wei-Zen Sun
§Department of Anesthesiology, College of Medicine, National Taiwan University, Taipei, Taiwan
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Yu-Hao Hu;
Yu-Hao Hu
*Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Taiwan
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Chi-An Chen;
Chi-An Chen
*Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Taiwan
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Wen-Fang Cheng
*Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Taiwan
‖Graduate Institute of Oncology, College of Medicine, National Taiwan University, Taipei, Taiwan
¶Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan
Correspondence: Professor Wen-Fang Cheng (email wenfangcheng@yahoo.com).
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Publisher: Portland Press Ltd
Received:
May 25 2011
Revision Received:
January 12 2012
Accepted:
January 23 2012
Accepted Manuscript online:
January 23 2012
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2012 Biochemical Society
2012
Clin Sci (Lond) (2012) 123 (6): 347–360.
Article history
Received:
May 25 2011
Revision Received:
January 12 2012
Accepted:
January 23 2012
Accepted Manuscript online:
January 23 2012
Citation
Ming-Cheng Chang, Chien-Nan Lee, Yu-Li Chen, Ying-Cheng Chiang, Wei-Zen Sun, Yu-Hao Hu, Chi-An Chen, Wen-Fang Cheng; Cord blood stem-cell-derived dendritic cells generate potent antigen-specific immune responses and anti-tumour effects. Clin Sci (Lond) 1 September 2012; 123 (6): 347–360. doi: https://doi.org/10.1042/CS20110272
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