The role of oxidative damage in the aetiology of coronary disease remains controversial, as clinical trials investigating the effect of antioxidants have not generally been positive. In the present study, 227 coronary cases, identified from a cohort study, were matched, by age and gender, with 420 controls in a nested case-control design. Stored plasma samples were analysed for F2-isoprostanes by stable isotope dilution MS, and specifically oxidized forms of apoA-I (apolipoprotein A-I) by HPLC of HDL (high-density lipoprotein). Median values of F2-isoprostanes were higher in plasma samples that contained oxidized apoA-I compared with samples with undetectable oxidized apoA-I (1542 compared with 1165 pmol/l). F2-Isoprostanes were significantly correlated with variants of non-oxidized apoA-II (r=−0.15) and were associated with HDL-cholesterol (P<0.0001). F2-Isoprostanes in cases (median, 1146 pmol/l) were not different from controls (1250 pmol/l); the odds ratio (95% confidence interval) for a 1 S.D. increase in F2-isoprostanes was 1.08 (0.91–1.29). Similarly, there was no independent association between the presence of oxidized apoA-I, detected in approx. 20% of the samples, and coronary risk. In conclusion, we found no evidence of associations between markers of lipid (F2-isoprostanes) and protein (oxidized apoA-I) oxidation and the risk of fatal or non-fatal coronary heart disease in a general population. This may be due to a true lack of association or insufficient power.
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Research Article|
November 28 2008
Association between both lipid and protein oxidation and the risk of fatal or non-fatal coronary heart disease in a human population
Mark Woodward;
*Department of Medicine, Mount Sinai Medical Center, New York, NY 10029-6574, U.S.A.
†The George Institute for International Health, University of Sydney, Camperdown, NSW 2050, Australia
Correspondence: Professor Mark Woodward (email mark.woodward@mountsinai.org).
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Kevin D. Croft;
Kevin D. Croft
‡School of Medicine and Pharmacology, University of Western Australia, Perth, WA 6001, Australia
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Trevor A. Mori;
Trevor A. Mori
‡School of Medicine and Pharmacology, University of Western Australia, Perth, WA 6001, Australia
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Henrietta Headlam;
Henrietta Headlam
‡School of Medicine and Pharmacology, University of Western Australia, Perth, WA 6001, Australia
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Xiao Suo Wang;
Xiao Suo Wang
§School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia
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Cacang Suarna;
Cacang Suarna
§School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia
∥School of Medical Sciences, Bosch Institute, Medical Foundation, University of Sydney, Camperdown, NSW 2050, Australia
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Mark J. Raftery;
Mark J. Raftery
¶Biomedical Mass Spectrometry Unit, University of New South Wales, Sydney, NSW 2052, Australia
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Stephen W. MacMahon;
Stephen W. MacMahon
†The George Institute for International Health, University of Sydney, Camperdown, NSW 2050, Australia
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Roland Stocker
Roland Stocker
§School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia
∥School of Medical Sciences, Bosch Institute, Medical Foundation, University of Sydney, Camperdown, NSW 2050, Australia
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Publisher: Portland Press Ltd
Received:
November 16 2007
Revision Received:
May 12 2008
Accepted:
May 28 2008
Accepted Manuscript online:
May 28 2008
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2009 Biochemical Society
2009
Clin Sci (Lond) (2009) 116 (1): 53–60.
Article history
Received:
November 16 2007
Revision Received:
May 12 2008
Accepted:
May 28 2008
Accepted Manuscript online:
May 28 2008
Connected Content
A commentary has been published:
More knocks to the oxidation hypothesis for vascular disease?
Citation
Mark Woodward, Kevin D. Croft, Trevor A. Mori, Henrietta Headlam, Xiao Suo Wang, Cacang Suarna, Mark J. Raftery, Stephen W. MacMahon, Roland Stocker; Association between both lipid and protein oxidation and the risk of fatal or non-fatal coronary heart disease in a human population. Clin Sci (Lond) 1 January 2009; 116 (1): 53–60. doi: https://doi.org/10.1042/CS20070404
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