Diabetes mellitus accelerates atherosclerotic processes, and it is known that inflammation plays a key role in atherosclerosis. The aim of the present study was to evaluate in patients with Type 2 diabetes whether serum levels of CRP (C-reactive protein) are associated with cytokine production in whole blood. A total of 89 outpatients with Type 2 diabetes were enrolled, and blood pressure, body mass index, fasting blood glucose, glycated haemoglobin, cholesterol, triacylglycerols (triglycerides) and hs-CRP (high-sensitivity CRP) were measured. IL-6 (interleukin-6), IL-1β (interleukin-1β) and TNF-α (tumour necrosis factor-α) were measured before and after 24 h of incubation of whole blood with LPS (lipopolysaccharide) or saline. The basal values of IL-1β, IL-6 and TNF-α were low and were not significantly related to hs-CRP levels. A univariate analysis showed that the level of IL-1β and IL-6, obtained after 24 h of incubation of whole blood with LPS, increased significantly with increasing levels of hs-CRP and, after adjusting for potential confounders, IL-1β still remained statistically significant. In our sample of patients with Type 2 diabetes, there was no association between serum hs-CRP levels and basal levels of IL-6, IL-1β and TNF-α. Conversely, a significant association was observed between serum hs-CRP levels and IL-1β and IL-6 production after 24 h of incubation of whole blood with LPS. In conclusion, our data suggest that patients with Type 2 diabetes and high hs-CRP levels may have an enhanced reactivity in response to specific stimuli that produce different interleukins, with possible implications in inflammatory atherosclerotic processes.
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July 2007
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Research Article|
June 13 2007
Association between serum values of C-reactive protein and cytokine production in whole blood of patients with Type 2 diabetes
Giovanna Castoldi;
*Clinica Nefrologica, Az. Ospedaliera San Gerardo, 20052 Monza, Italy
†Dipartimento di Medicina Clinica e Prevenzione, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
Correspondence: Dr Giovanna Castoldi, at the Dipartimento di Medicina Clinica e Prevenzione, Università degli Studi di Milano-Bicocca, Azienda Ospedaliera S.Gerardo di Monza, Via Pergolesi 33, 20052 Monza, Italy (email giovanna.castoldi@unimib.it).
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Stefania Galimberti;
Stefania Galimberti
†Dipartimento di Medicina Clinica e Prevenzione, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
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Chiara Riva;
Chiara Riva
‡Clinica Neurologica, Dipartimento di Neuroscienze, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
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Ruggero Papagna;
Ruggero Papagna
§Clinica Odontoiatrica, Dipartimento di Neuroscienze, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
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Federico Querci;
Federico Querci
∥Centro di Diabetologia, Ospedale di Alzano Lombardo, Azienda Bolognini, 24068 Seriate, Italy
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Marco Casati;
Marco Casati
*Clinica Nefrologica, Az. Ospedaliera San Gerardo, 20052 Monza, Italy
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Gianpaolo Zerbini;
Gianpaolo Zerbini
¶Unita' di Fisiopatologia Renale, Sezione Nutrizione-Metabolismo, Medicina, Istituto Scientifico San Raffaele, 20132 Milan, Italy
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Gianluigi Caccianiga;
Gianluigi Caccianiga
§Clinica Odontoiatrica, Dipartimento di Neuroscienze, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
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Carlo Ferrarese;
Carlo Ferrarese
‡Clinica Neurologica, Dipartimento di Neuroscienze, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
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Marco Baldoni;
Marco Baldoni
§Clinica Odontoiatrica, Dipartimento di Neuroscienze, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
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Maria Grazia Valsecchi;
Maria Grazia Valsecchi
†Dipartimento di Medicina Clinica e Prevenzione, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
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Andrea Stella
Andrea Stella
*Clinica Nefrologica, Az. Ospedaliera San Gerardo, 20052 Monza, Italy
†Dipartimento di Medicina Clinica e Prevenzione, Università degli Studi di Milano-Bicocca, 20052 Monza, Italy
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Publisher: Portland Press Ltd
Received:
November 29 2006
Revision Received:
March 01 2007
Accepted:
March 16 2007
Accepted Manuscript online:
March 16 2007
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2007 The Biochemical Society
2007
Clin Sci (Lond) (2007) 113 (2): 103–108.
Article history
Received:
November 29 2006
Revision Received:
March 01 2007
Accepted:
March 16 2007
Accepted Manuscript online:
March 16 2007
Citation
Giovanna Castoldi, Stefania Galimberti, Chiara Riva, Ruggero Papagna, Federico Querci, Marco Casati, Gianpaolo Zerbini, Gianluigi Caccianiga, Carlo Ferrarese, Marco Baldoni, Maria Grazia Valsecchi, Andrea Stella; Association between serum values of C-reactive protein and cytokine production in whole blood of patients with Type 2 diabetes. Clin Sci (Lond) 1 July 2007; 113 (2): 103–108. doi: https://doi.org/10.1042/CS20060338
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