The objective of this study was to identify cellular and plasma marker(s) of post-I/R (ischaemia/reperfusion) in patients undergoing elective knee surgery where a tourniquet was used to facilitate a bloodless surgical field. We evaluated the inflammatory and redox response by measuring the mRNA levels of ICAM-1 (intercellular cell-adhesion molecule-1), MnSOD (manganese superoxide dismutase), GST-μ (glutathione transferase-μ) and Cu/ZnSOD (copper/zinc superoxide dismutase) in the operated muscle and blood cells pre-operatively (pre-tourniquet) and at various times after reperfusion (tourniquet release). We also measured plasma concentrations of IL (interleukin)-6, IL-8, sICAM-1 (soluble ICAM-1), IL-1β and TNF-α (tumour necrosis factor-α) using ELISA. Our results show a strong induction of MnSOD and GST-μ in granulocytes (but not in mononuclear cells or muscle) after reperfusion (2 and 4 h). There was no change in the mRNA level of Cu/ZnSOD after reperfusion. An up-regulation of membrane ICAM-1 in muscle and a decrease in sICAM-1 in plasma were detected after reperfusion. Plasma IL-6 and IL-8 levels (but not TNF-α or IL-1β) increased significantly over baseline at 2 and 4 h after reperfusion. Elevated expression of ICAM-1 in muscle, MnSOD and GST-μ in granulocytes and increased levels of plasma IL-6 and IL-8 may be considered as phase- and cell-specific markers of post-I/R of skeletal muscle in humans.
Skip Nav Destination
Article navigation
November 2004
-
Cover Image
Cover Image
- PDF Icon PDF LinkTable of Contents
Research Article|
October 26 2004
Inflammatory and redox responses to ischaemia/reperfusion in human skeletal muscle
Ruksana HUDA;
Ruksana HUDA
1Department of Anesthesiology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0591, U.S.A.
Search for other works by this author on:
Daneshvari R. SOLANKI;
Daneshvari R. SOLANKI
1Department of Anesthesiology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0591, U.S.A.
Search for other works by this author on:
Mali MATHRU
1Department of Anesthesiology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0591, U.S.A.
Correspondence: Dr Mali Mathru (email rhuda@utmb.edu).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
June 21 2004
Revision Received:
July 23 2004
Accepted:
July 29 2004
Accepted Manuscript online:
July 29 2004
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society
2004
Clin Sci (Lond) (2004) 107 (5): 497–503.
Article history
Received:
June 21 2004
Revision Received:
July 23 2004
Accepted:
July 29 2004
Accepted Manuscript online:
July 29 2004
Citation
Ruksana HUDA, Daneshvari R. SOLANKI, Mali MATHRU; Inflammatory and redox responses to ischaemia/reperfusion in human skeletal muscle. Clin Sci (Lond) 1 November 2004; 107 (5): 497–503. doi: https://doi.org/10.1042/CS20040179
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.