Inflammatory diseases are associated with reduced serum concentrations of α2-HS glycoprotein (the human homologue of bovine fetuin), but the role of fetuin in inflammation is poorly understood. We hypothesized that fetuin may influence the resolution of inflammation by modulating the phagocytosis of apoptotic cells by macrophages. Using an in vitro flow cytometry-based phagocytosis assay, we investigated the role of fetuin in apoptotic cell clearance. Bovine fetuin and human α2-HS glycoprotein significantly augmented the phagocytosis of apoptotic cells by human peripheral blood monocyte-derived macrophages, whereas the control proteins BSA, sialylated BSA and asialofetuin were ineffective. The enhancement of phagocytosis was concentration-dependent, and required the presence of intact fetuin at the time of interaction between macrophages and apoptotic cells. Fetuin also substantially increased the uptake of labelled dextran 70000 by macrophages, which occurs by macropinocytosis, suggesting that this may be one of the mechanisms utilized for apoptotic cell uptake.
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September 2003
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Research Article|
September 01 2003
Fetuin/α2-HS glycoprotein enhances phagocytosis of apoptotic cells and macropinocytosis by human macrophages
Hubertus P. A. JERSMANN;
Hubertus P. A. JERSMANN
1MRC Centre for Inflammation Research, University of Edinburgh Medical School, Teviot Place, Edinburgh EH8 9AG, U.K.
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Ian DRANSFIELD;
Ian DRANSFIELD
1MRC Centre for Inflammation Research, University of Edinburgh Medical School, Teviot Place, Edinburgh EH8 9AG, U.K.
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Simon P. HART
1MRC Centre for Inflammation Research, University of Edinburgh Medical School, Teviot Place, Edinburgh EH8 9AG, U.K.
Correspondence: Dr S. P. Hart (e-mail s.hart@ed.ac.uk).
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Publisher: Portland Press Ltd
Received:
April 08 2003
Revision Received:
April 22 2003
Accepted:
May 02 2003
Accepted Manuscript online:
May 02 2003
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2003 The Biochemical Society
2003
Clin Sci (Lond) (2003) 105 (3): 273–278.
Article history
Received:
April 08 2003
Revision Received:
April 22 2003
Accepted:
May 02 2003
Accepted Manuscript online:
May 02 2003
Citation
Hubertus P. A. JERSMANN, Ian DRANSFIELD, Simon P. HART; Fetuin/α2-HS glycoprotein enhances phagocytosis of apoptotic cells and macropinocytosis by human macrophages. Clin Sci (Lond) 1 September 2003; 105 (3): 273–278. doi: https://doi.org/10.1042/CS20030126
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