We report here that monounsaturated fatty acids and polyunsaturated fatty acids (PUFAs) provoke the accumulation of neutral lipids and apoptosis in retinoic acid-treated HL-60 cells in a concentration- and time-dependent manner. The PUFAs (arachidonic acid, docosahexanoic acid and eicosapentaenoic acid) provoked higher levels of HL-60 apoptosis compared with the monounsaturated oleic acid or the saturated palmitic acid. Cell size and granularity were also altered by fatty acid treatment. The PUFA-induced apoptosis was correlated with increased activity of caspase 3 and caspase 9. Lipid peroxidation was also increased in the presence of PUFAs, but was not responsible for activating cell apoptosis. Lipid derived metabolites may be responsible for activation of caspases and induction of cell apoptosis.
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Research Article|
January 17 2003
Polyunsaturated and monounsaturated fatty acids increase neutral lipid accumulation, caspase activation and apoptosis in a neutrophil-like, differentiated HL-60 cell line
Declan A. HEALY;
Declan A. HEALY
*Department of Biochemistry, University College Dublin, Belfield, Dublin 4, Ireland
†Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
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R. William G. WATSON;
R. William G. WATSON
†Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
‡Department of Surgery, Mater Misericordiae Hospital, University College Dublin, 47 Eccles Street, Dublin 7, Ireland
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Philip NEWSHOLME
*Department of Biochemistry, University College Dublin, Belfield, Dublin 4, Ireland
†Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
Correspondence: Dr Philip Newsholme (e-mail philip.newsholme@ucd.ie).
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Publisher: Portland Press Ltd
Received:
August 13 2002
Accepted:
November 26 2002
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 2003
2003
Clin Sci (Lond) (2003) 104 (2): 171–179.
Article history
Received:
August 13 2002
Accepted:
November 26 2002
Citation
Declan A. HEALY, R. William G. WATSON, Philip NEWSHOLME; Polyunsaturated and monounsaturated fatty acids increase neutral lipid accumulation, caspase activation and apoptosis in a neutrophil-like, differentiated HL-60 cell line. Clin Sci (Lond) 1 February 2003; 104 (2): 171–179. doi: https://doi.org/10.1042/cs1040171
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