To examine the reproducibility of the hyperinsulinaemic–euglycaemic clamp technique at mid-physiological hyperinsulinaemia, seven healthy subjects {age 50 (25, 59) years [median (range)], body mass index 23.1 (20.8, 25.5) kg·m-2} were investigated with three 2 h hyperinsulinaemic (60 µmol·l-1)–euglycaemic (4.5 mmol·l-1) clamps performed 48 h and 14 days apart respectively. The third clamp was prolonged to 8 h in order to examine effects on glucose disposal during prolonged clamps. The glucose infusion rates (GIRs) during the three 2 h clamps were 7.41 (4.28, 10.96), 7.26 (5.38, 11.02) and 6.63 (4.42, 10.3) mg·kg-1·min-1, with a median intra-individual coefficient of variation of 5.8 (2.6, 22) %. During the 8 h clamp a highly variable gradual increase in GIR was observed, reaching a plateau between 4 and 5 h at 32 (5, 101) % above the GIR between 1 and 2 h (P < 0.05). This increase was correlated inversely with the GIR between 1 and 2 h (r = -0.82; P < 0.05), and directly with age (r = 0.86; P < 0.05). Carbohydrate oxidation measured by indirect calorimetry was stable during the repeated 2 h clamps and the 8 h clamp. Endogenous glucose production measured by infusion of [6,6-2H2]glucose was suppressed during the 8 h clamp. The 2 h hyperinsulinaemic–euglycaemic clamp is reproducible at a mid-physiological range of hyperinsulinaemia. If prolonged, it results in a delayed increase in non-oxidative glucose disposal, which is most pronounced in subjects with low insulin sensitivity. The findings underline the importance of selecting age-matched controls in studies of insulin resistance.
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Research Article|
February 24 2000
The hyperinsulinaemic–euglycaemic glucose clamp: reproducibility and metabolic effects of prolonged insulin infusion in healthy subjects
Mattias SOOP;
Mattias SOOP
1
*Department of Surgery, Karolinska Hospital, S-171 76 Stockholm, Sweden
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Jonas NYGREN;
Jonas NYGREN
2
*Department of Surgery, Karolinska Hospital, S-171 76 Stockholm, Sweden
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Kerstin BRISMAR;
Kerstin BRISMAR
‡Department of Endocrinology, Karolinska Hospital, S-171 76 Stockholm, Sweden
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Anders THORELL;
Anders THORELL
3
†Department of Surgery, Karolinska Institute at Huddinge University Hospital, S-141 86 Huddinge, Sweden
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Olle LJUNGQVIST
Olle LJUNGQVIST
4
†Department of Surgery, Karolinska Institute at Huddinge University Hospital, S-141 86 Huddinge, Sweden
Correspondence: Dr Olle Ljungqvist (e-mail olllju@mbox.ki.se).
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Publisher: Portland Press Ltd
Received:
August 26 1999
Accepted:
November 16 1999
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 2000
2000
Clin Sci (Lond) (2000) 98 (4): 367–374.
Article history
Received:
August 26 1999
Accepted:
November 16 1999
Citation
Mattias SOOP, Jonas NYGREN, Kerstin BRISMAR, Anders THORELL, Olle LJUNGQVIST; The hyperinsulinaemic–euglycaemic glucose clamp: reproducibility and metabolic effects of prolonged insulin infusion in healthy subjects. Clin Sci (Lond) 1 April 2000; 98 (4): 367–374. doi: https://doi.org/10.1042/cs0980367
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