Stable-isotope tracers were used to assess whether levels of phosphomonoesters (PME) and phosphodiesters (PDE) in the livers of lung cancer patients, as observed by 31P magnetic resonance (MR) spectroscopy, reflect elevated whole-body glucose turnover and gluconeogenesis from alanine. Patients with advanced non-small-cell lung cancer without liver metastases (n = 24; weight loss 0–24%) and healthy control subjects (n = 13) were studied after an overnight fast. 31P MR spectra of the liver in vivo were obtained, and glucose turnover and gluconeogenesis from alanine were determined simultaneously using primed-constant infusions of [6,6-2H2]glucose and [3-13C]alanine. Liver PME concentrations were 6% higher in lung cancer patients compared with controls (not significant); PME levels in patients with ⩾ 5% weight loss were significantly higher than in patients with < 5% weight loss (P < 0.01). PDE levels did not differ between the groups. In lung cancer patients, whole-body glucose production was 19% higher (not significant) and gluconeogenesis from alanine was 42% higher (P < 0.05) compared with healthy subjects; turnover rates in lung cancer patients with ⩾ 5% weight loss were significantly elevated compared with both patients with < 5% weight loss and healthy subjects (P < 0.05). PME levels were significantly correlated with glucose turnover and gluconeogenesis from alanine in lung cancer patients (r = 0.48 and r = 0.48 respectively; P < 0.05). In conclusion, elevated PME levels in lung cancer patients appear to reflect increased glucose flux and gluconeogenesis from alanine. These results are consistent with the hypothesis that elevated PME levels are due to contributions from gluconeogenic intermediates.
Skip Nav Destination
Article navigation
Research Article|
January 19 2000
Hepatic sugar phosphate levels reflect gluconeogenesis in lung cancer: simultaneous turnover measurements and 31P magnetic resonance spectroscopy in vivo
Susanne LEIJ-HALFWERK;
Susanne LEIJ-HALFWERK
*Institute of Internal Medicine II, University Hospital Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
†Department of Diagnostic Radiology, University Hospital Rotterdam, P.O. Box 5201, 3008 AE Rotterdam, The Netherlands
Search for other works by this author on:
Pieter C. DAGNELIE;
*Institute of Internal Medicine II, University Hospital Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
‡Department of Epidemiology, University of Maastricht, P.O. Box 616, 6200 MD Maastricht, The Netherlands
Correspondence: Dr P. C. Dagnelie, at Department of Epidemiology, University of Maastricht (e-mail dagnelie@epid.unimaas.nl).
Search for other works by this author on:
J. Willem O. VAN DEN BERG;
J. Willem O. VAN DEN BERG
*Institute of Internal Medicine II, University Hospital Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
Search for other works by this author on:
J. H. Paul WILSON;
J. H. Paul WILSON
*Institute of Internal Medicine II, University Hospital Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
Search for other works by this author on:
Paul E. SIJENS
Paul E. SIJENS
†Department of Diagnostic Radiology, University Hospital Rotterdam, P.O. Box 5201, 3008 AE Rotterdam, The Netherlands
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
July 08 1999
Revision Received:
September 08 1999
Accepted:
October 15 1999
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society and the Medical Research Society © 2000
2000
Clin Sci (Lond) (2000) 98 (2): 167–174.
Article history
Received:
July 08 1999
Revision Received:
September 08 1999
Accepted:
October 15 1999
Citation
Susanne LEIJ-HALFWERK, Pieter C. DAGNELIE, J. Willem O. VAN DEN BERG, J. H. Paul WILSON, Paul E. SIJENS; Hepatic sugar phosphate levels reflect gluconeogenesis in lung cancer: simultaneous turnover measurements and 31P magnetic resonance spectroscopy in vivo. Clin Sci (Lond) 1 February 2000; 98 (2): 167–174. doi: https://doi.org/10.1042/cs0980167
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.