A predisposing factor for development of the hyperglycaemic state of gestational diabetes mellitus (GDM) is obesity. We previously showed that increasing maternal obesity is associated with significant reductions in placental mitochondrial respiration. MicroRNA (miR)-143 has been previously shown to regulate the metabolic switch from oxidative phosphorylation to aerobic glycolysis in cancer tissues. We hypothesized that mitochondrial respiration is reduced and aerobic glycolysis is up-regulated via changes in miR-143 expression in the placenta of women with GDM. Placental tissue was collected at term from women with A1GDM (controlled by diet), A2GDM (controlled by medication) and body mass index (BMI)-matched controls (CTRL). miR-143 expression was measured by RT-PCR. Expression of mitochondrial complexes, transcription factors peroxisome proliferator-activated receptor-γ co-activator 1α (PGC1α) and peroxisome proliferator-activated receptor γ (PPARγ), components of mammalian target of rapamycin (mTOR) signalling, glucose transporter GLUT1 and glycolytic enzymes [hexokinase-2 (HK-2), phosphofructokinase (PFK) and lactate dehydrogenase (LDH)] were measured by Western blot. Trophoblast respiration was measured by XF24 Analyser. Expression of miR-143, mitochondrial complexes, and PPARγ and PGC1α, which act downstream of miR-143, were significantly decreased in A2GDM placentae compared with A1GDM and CTRL (P<0.01). Placental hPL (human placental lactogen) levels, expression of glycolytic enzymes, GLUT1 and mTOR signalling were also significantly increased by more than 2-fold in A2GDM compared with A1GDM and CTRL (P<0.05). There was a 50% reduction in mitochondrial respiration in trophoblast cells isolated from A2GDM placentae. Overexpression of miR-143 was able to increase mitochondrial respiration, increase protein expression of mitochondrial complexes and decrease expression of glycolytic enzymes by 40% compared with A2GDM. Down-regulation of miR-143 mediates the metabolic switch from oxidative phosphorylation to aerobic glycolysis in placenta of women with A2GDM.
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June 2016
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Mitochondrial protein quality control plays a decisive role in the maintenance of a proper mitochondrial function in metabolically active tissues such as skeletal muscle. Several chaperones and proteases, involved in the regulation of mitochondrial protein quality control, are altered in obesity and type 2 diabetes mellitus. For further details see Dahlmans et al. pp. 843–852.
Research Article|
April 29 2016
Mitochondrial function and glucose metabolism in the placenta with gestational diabetes mellitus: role of miR-143
Sribalasubashini Muralimanoharan;
Sribalasubashini Muralimanoharan
1
*Center for Pregnancy and Newborn Research, Department of Ob/Gyn, University of Texas Health Science Center San Antonio, San Antonio, TX 78229, U.S.A.
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Alina Maloyan;
Alina Maloyan
2
*Center for Pregnancy and Newborn Research, Department of Ob/Gyn, University of Texas Health Science Center San Antonio, San Antonio, TX 78229, U.S.A.
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Leslie Myatt
Leslie Myatt
3
*Center for Pregnancy and Newborn Research, Department of Ob/Gyn, University of Texas Health Science Center San Antonio, San Antonio, TX 78229, U.S.A.
Correspondence: Leslie Myatt (email myattl@ohsu.edu).
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Publisher: Portland Press Ltd
Received:
March 03 2016
Accepted:
March 17 2016
Accepted Manuscript online:
March 31 2016
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society
2016
Clin Sci (Lond) (2016) 130 (11): 931–941.
Article history
Received:
March 03 2016
Accepted:
March 17 2016
Accepted Manuscript online:
March 31 2016
Citation
Sribalasubashini Muralimanoharan, Alina Maloyan, Leslie Myatt; Mitochondrial function and glucose metabolism in the placenta with gestational diabetes mellitus: role of miR-143. Clin Sci (Lond) 1 June 2016; 130 (11): 931–941. doi: https://doi.org/10.1042/CS20160076
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