Exercise and hypoxia paradoxically modulate vascular thrombotic risks. The shedding of procoagulant-rich microparticles from monocytes may accelerate the pathogenesis of atherothrombosis. The present study explores the manner in which normoxic and hypoxic exercise regimens affect procoagulant monocyte-derived microparticle (MDMP) formation and monocyte-promoted thrombin generation (TG). Forty sedentary healthy males were randomized to perform either normoxic (NET; 21% O2, n=20) or hypoxic (HET; 15% O2, n=20) exercise training (60% VO2max) for 30 min/day, 5 days/week for 5 weeks. At rest and immediately after HET (100 W under 12% O2 for 30 min), the MDMP characteristics and dynamic TG were measured by flow cytometry and thrombinography respectively. The results demonstrated that acute 12% O2 exercise (i) increased the release of coagulant factor V (FV)/FVIII-rich, phosphatidylserine (PS)-exposed and tissue factor (TF)-expressed microparticles from monocytes, (ii) enhanced the peak height and rate of TG in monocyte-rich plasma (MRP) and (iii) elevated concentrations of norepinephrine/epinephrine, myeloperoxidase (MPO) and interleukin-6 (IL-6) in plasma. Following the 5-week intervention, HET exhibited higher enhancements of peak work-rate and cardiopulmonary fitness than NET did. Moreover, both NET and HET decreased the FV/FVIII-rich, PS-exposed and TF-expressed MDMP counts and the peak height and rate of TG in MRP following the HET. However, HET elicited more suppression for the HE (hypoxic exercise)-enhanced procoagulant MDMP formation and dynamic TG in MPR and catecholamine/peroxide/pro-inflammatory cytokine levels in plasma than NET. Hence, we conclude that HET is superior to NET for enhancing aerobic capacity. Furthermore, HET effectively suppresses procoagulant MDMP formation and monocyte-mediated TG under severe hypoxic stress, compared with NET.
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Research Article|
May 27 2015
Effects of normoxic and hypoxic exercise regimens on monocyte-mediated thrombin generation in sedentary men
Jong-Shyan Wang;
*Healthy Aging Research Center, Graduate Institute of Rehabilitation Science, Medical Collage, Chang Gung University, Tao-Yuan 333, Taiwan
†Heart Failure Center, Chang Gung Memorial Hospital, Keelung 204, Taiwan
Correspondence: Professor Jong-Shyan Wang (email s5492@mail.cgu.edu.tw).
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Ya-Lun Chang;
Ya-Lun Chang
*Healthy Aging Research Center, Graduate Institute of Rehabilitation Science, Medical Collage, Chang Gung University, Tao-Yuan 333, Taiwan
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Yi-Ching Chen;
Yi-Ching Chen
*Healthy Aging Research Center, Graduate Institute of Rehabilitation Science, Medical Collage, Chang Gung University, Tao-Yuan 333, Taiwan
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Hsing-Hua Tsai;
Hsing-Hua Tsai
*Healthy Aging Research Center, Graduate Institute of Rehabilitation Science, Medical Collage, Chang Gung University, Tao-Yuan 333, Taiwan
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Tieh-Cheng Fu
Tieh-Cheng Fu
†Heart Failure Center, Chang Gung Memorial Hospital, Keelung 204, Taiwan
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Publisher: Portland Press Ltd
Received:
February 11 2015
Revision Received:
March 24 2015
Accepted:
March 31 2015
Accepted Manuscript online:
March 31 2015
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© 2015 Authors; published by Portland Press Limited
2015
Clin Sci (Lond) (2015) 129 (4): 363–374.
Article history
Received:
February 11 2015
Revision Received:
March 24 2015
Accepted:
March 31 2015
Accepted Manuscript online:
March 31 2015
Citation
Jong-Shyan Wang, Ya-Lun Chang, Yi-Ching Chen, Hsing-Hua Tsai, Tieh-Cheng Fu; Effects of normoxic and hypoxic exercise regimens on monocyte-mediated thrombin generation in sedentary men. Clin Sci (Lond) 1 August 2015; 129 (4): 363–374. doi: https://doi.org/10.1042/CS20150128
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