This study has demonstrated for the first time that cryopreservation of primary immune cells modified their metabolism in a time-dependent fashion, indicated by attenuated aerobic respiration and enhanced glycolytic activity. Measurements were recorded using the Seahorse XFe96 extracellular flux analyser.

Keywords:
bioenergetics, cryopreservation, leucocytes, peripheral blood mononuclear cells, polymorphonuclear cells inflammation
© The Authors Journal compilation © 2015 Biochemical Society
2015
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