Although AngII (angiotensin II) and its receptor AT1R (AngII type 1 receptor) have been implicated in AAA (abdominal aortic aneurysm) formation, the proximal signalling events primarily responsible for AAA formation remain uncertain. Caveolae are cholesterol-rich membrane microdomains that serve as a signalling platform to facilitate the temporal and spatial localization of signal transduction events, including those stimulated by AngII. Cav1 (caveolin 1)-enriched caveolae in vascular smooth muscle cells mediate ADAM17 (a disintegrin and metalloproteinase 17)-dependent EGFR (epidermal growth factor receptor) transactivation, which is linked to vascular remodelling induced by AngII. In the present study, we have tested our hypothesis that Cav1 plays a critical role for the development of AAA at least in part via its specific alteration of AngII signalling within caveolae. Cav1−/− mice and the control wild-type mice were co-infused with AngII and β-aminopropionitrile to induce AAA. We found that Cav1−/− mice with the co-infusion did not develop AAA compared with control mice in spite of hypertension. We found an increased expression of ADAM17 and enhanced phosphorylation of EGFR in AAA. These events were markedly attenuated in Cav1−/− aortas with the co-infusion. Furthermore, aortas from Cav1−/− mice with the co-infusion showed less endoplasmic reticulum stress, oxidative stress and inflammatory responses compared with aortas from control mice. Cav1 silencing in cultured vascular smooth muscle cells prevented AngII-induced ADAM17 induction and activation. In conclusion, Cav1 appears to play a critical role in the formation of AAA and associated endoplasmic reticulum/oxidative stress, presumably through the regulation of caveolae compartmentalized signals induced by AngII.
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Research Article|
February 13 2014
Caveolin 1 is critical for abdominal aortic aneurysm formation induced by angiotensin II and inhibition of lysyl oxidase
Takehiko Takayanagi;
Takehiko Takayanagi
1
*Cardiovascular Research Center, Department of Physiology, Temple University School of Medicine, Philadelphia, PA 19140, U.S.A.
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Kevin J. Crawford;
Kevin J. Crawford
1
*Cardiovascular Research Center, Department of Physiology, Temple University School of Medicine, Philadelphia, PA 19140, U.S.A.
†Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, PA 19140, U.S.A.
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Tomonori Kobayashi;
Tomonori Kobayashi
*Cardiovascular Research Center, Department of Physiology, Temple University School of Medicine, Philadelphia, PA 19140, U.S.A.
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Takashi Obama;
Takashi Obama
*Cardiovascular Research Center, Department of Physiology, Temple University School of Medicine, Philadelphia, PA 19140, U.S.A.
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Toshiyuki Tsuji;
Toshiyuki Tsuji
*Cardiovascular Research Center, Department of Physiology, Temple University School of Medicine, Philadelphia, PA 19140, U.S.A.
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Katherine J. Elliott;
Katherine J. Elliott
*Cardiovascular Research Center, Department of Physiology, Temple University School of Medicine, Philadelphia, PA 19140, U.S.A.
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Tomoki Hashimoto;
Tomoki Hashimoto
‡Department of Anesthesia and Perioperative Care, University of California, San Francisco, San Francisco, CA 94110, U.S.A.
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Victor Rizzo;
*Cardiovascular Research Center, Department of Physiology, Temple University School of Medicine, Philadelphia, PA 19140, U.S.A.
†Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, PA 19140, U.S.A.
Correspondence: Dr Victor Rizzo (rizzov@temple.edu) or Professor Satoru Eguchi (email seguchi@temple.edu).
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Satoru Eguchi
*Cardiovascular Research Center, Department of Physiology, Temple University School of Medicine, Philadelphia, PA 19140, U.S.A.
Correspondence: Dr Victor Rizzo (rizzov@temple.edu) or Professor Satoru Eguchi (email seguchi@temple.edu).
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Publisher: Portland Press Ltd
Received:
October 16 2013
Revision Received:
December 12 2013
Accepted:
December 13 2013
Accepted Manuscript online:
December 13 2013
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2014 Biochemical Society
2014
Clin Sci (Lond) (2014) 126 (11): 785–800.
Article history
Received:
October 16 2013
Revision Received:
December 12 2013
Accepted:
December 13 2013
Accepted Manuscript online:
December 13 2013
Citation
Takehiko Takayanagi, Kevin J. Crawford, Tomonori Kobayashi, Takashi Obama, Toshiyuki Tsuji, Katherine J. Elliott, Tomoki Hashimoto, Victor Rizzo, Satoru Eguchi; Caveolin 1 is critical for abdominal aortic aneurysm formation induced by angiotensin II and inhibition of lysyl oxidase. Clin Sci (Lond) 1 June 2014; 126 (11): 785–800. doi: https://doi.org/10.1042/CS20130660
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