In the present study, we have investigated the possible changes in rat mesenteric artery vascular innervation function caused by chronic exposure to low doses of HgCl2 (mercuric chloride), as well as the mechanisms involved. Rats were divided into two groups: (i) control, and (ii) HgCl2-treated rats (30 days; first dose, 4.6 μg/kg of body weight; subsequent dose, 0.07 μg·kg−1 of body weight·day−1, intramuscularly). Vasomotor response to EFS (electrical field stimulation), NA (noradrenaline) and the NO donor DEA-NO (diethylamine NONOate) were studied, nNOS (neuronal NO synthase) and phospho-nNOS protein expression were analysed, and NO, O2− (superoxide anion) and NA release were also determined. EFS-induced contraction was higher in the HgCl2-treated group. Phentolamine (1 μmol/l) decreased the response to EFS to a greater extent in HgCl2-treated rats. HgCl2 treatment increased vasoconstrictor response to exogenous NA and NA release. L-NAME (NG-nitro-L-arginine methyl ester; 0.1 mmol/l) increased the response to EFS in both experimental groups, but the increase was greater in segments from control animals. HgCl2 treatment decreased NO release and increased O2− production. Vasodilator response to DEA-NO was lower in HgCl2-treated animals. Tempol increased DEA-NO-induced relaxation to a greater extent in HgCl2-treated animals. nNOS expression was similar in arteries from both experimental groups, whereas phospho-nNOS was decreased in segments from HgCl2-treated animals. HgCl2 treatment increased vasoconstrictor response to EFS as a result of, in part, reduced NO bioavailability and increased adrenergic function. These findings offer further evidence that mercury, even at low concentrations, is an environmental risk factor for cardiovascular disease.
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Research Article|
June 24 2011
Chronic HgCl2 treatment increases vasoconstriction induced by electrical field stimulation: role of adrenergic and nitrergic innervation
Javier Blanco-Rivero;
Javier Blanco-Rivero
*Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain
†Instituto de Investigación Sanitaria (IdiPAZ), Hospital Universitario ‘La Paz’, Madrid, Spain
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Lorena B. Furieri;
Lorena B. Furieri
‡Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain
§Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, ES, Brazil
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Dalton V. Vassallo;
Dalton V. Vassallo
§Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, ES, Brazil
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Mercedes Salaices;
Mercedes Salaices
‡Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain
§Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, ES, Brazil
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Gloria Balfagón
*Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain
†Instituto de Investigación Sanitaria (IdiPAZ), Hospital Universitario ‘La Paz’, Madrid, Spain
Correspondence: Professor Gloria Balfagón (email gloria.balfagon@uam.es).
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Publisher: Portland Press Ltd
Received:
February 08 2011
Revision Received:
April 26 2011
Accepted:
May 09 2011
Accepted Manuscript online:
May 09 2011
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2011 Biochemical Society
2011
Clin Sci (Lond) (2011) 121 (8): 331–341.
Article history
Received:
February 08 2011
Revision Received:
April 26 2011
Accepted:
May 09 2011
Accepted Manuscript online:
May 09 2011
Citation
Javier Blanco-Rivero, Lorena B. Furieri, Dalton V. Vassallo, Mercedes Salaices, Gloria Balfagón; Chronic HgCl2 treatment increases vasoconstriction induced by electrical field stimulation: role of adrenergic and nitrergic innervation. Clin Sci (Lond) 1 October 2011; 121 (8): 331–341. doi: https://doi.org/10.1042/CS20110072
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