End-stage renal disease patients have endothelial dysfunction and high plasma levels of ADMA (asymmetric ω-NG,NG-dimethylarginine), an endogenous inhibitor of NOS (NO synthase). The actual link between these abnormalities is controversial. Therefore, in the present study, we investigated whether HD (haemodialysis) has an acute impact on NO-dependent vasodilation and plasma ADMA in these patients. A total of 24 patients undergoing maintenance HD (HD group) and 24 age- and gender-matched healthy controls (Control group) were enrolled. The increase in forearm SkBF (skin blood flow) caused by local heating to 41 °C (SkBF41), known to depend on endothelial NO production, was determined with laser Doppler imaging. SkBF41 was expressed as a percentage of the vasodilatory reserve obtained from the maximal SkBF induced by local heating to 43 °C (independent of NO). In HD patients, SkBF41 was assessed on two successive HD sessions, once immediately before and once immediately after HD. Plasma ADMA was assayed simultaneously with MS/MS (tandem MS). In the Control group, SkBF41 was determined twice, on two different days, and plasma ADMA was assayed once. In HD patients, SkBF41 was identical before (82.2±13.1%) and after (82.7±12.4%) HD, but was lower than in controls (day 1, 89.6±6.1; day 2, 89.2±6.9%; P<0.01 compared with the HD group). In contrast, plasma ADMA was higher before (0.98±0.17 μmol/l) than after (0.58±0.10 μmol/l; P<0.01) HD. ADMA levels after HD did not differ from those obtained in controls (0.56±0.11 μmol/l). These findings show that HD patients have impaired NO-dependent vasodilation in forearm skin, an abnormality not acutely reversed by HD and not explained by ADMA accumulation.
Skip Nav Destination
Article navigation
Research Article|
August 24 2009
Haemodialysis acutely reduces the plasma levels of ADMA without reversing impaired NO-dependent vasodilation
Rolf P. Engelberger;
Rolf P. Engelberger
*Division de Physiopathologie Clinique, Centre Hospitalier Universitaire Vaudois (CHUV) et Université de Lausanne (UNIL), BH10-701 Lausanne, Switzerland
Search for other works by this author on:
Daniel Teta;
Daniel Teta
†Service de Néphrologie, Centre Hospitalier Universitaire Vaudois (CHUV) et Université de Lausanne (UNIL), BH10-701 Lausanne, Switzerland
Search for other works by this author on:
Hughes Henry;
Hughes Henry
‡Laboratoire Central de Chimie Clinique, Centre Hospitalier Universitaire Vaudois (CHUV) et Université de Lausanne (UNIL), BH10-701 Lausanne, Switzerland
Search for other works by this author on:
Olivier De Senarclens;
Olivier De Senarclens
*Division de Physiopathologie Clinique, Centre Hospitalier Universitaire Vaudois (CHUV) et Université de Lausanne (UNIL), BH10-701 Lausanne, Switzerland
Search for other works by this author on:
Benoît Dischl;
Benoît Dischl
*Division de Physiopathologie Clinique, Centre Hospitalier Universitaire Vaudois (CHUV) et Université de Lausanne (UNIL), BH10-701 Lausanne, Switzerland
Search for other works by this author on:
Lucas Liaudet;
Lucas Liaudet
§Soins Intensifs de Médecine Adulte, Centre Hospitalier Universitaire Vaudois (CHUV) et Université de Lausanne (UNIL), BH10-701 Lausanne, Switzerland
Search for other works by this author on:
Michel Burnier;
Michel Burnier
†Service de Néphrologie, Centre Hospitalier Universitaire Vaudois (CHUV) et Université de Lausanne (UNIL), BH10-701 Lausanne, Switzerland
Search for other works by this author on:
Bernard Waeber;
Bernard Waeber
*Division de Physiopathologie Clinique, Centre Hospitalier Universitaire Vaudois (CHUV) et Université de Lausanne (UNIL), BH10-701 Lausanne, Switzerland
Search for other works by this author on:
François Feihl
*Division de Physiopathologie Clinique, Centre Hospitalier Universitaire Vaudois (CHUV) et Université de Lausanne (UNIL), BH10-701 Lausanne, Switzerland
Correspondence: Dr François Feihl (email Francois.Feihl@chuv.ch).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
October 29 2008
Revision Received:
February 13 2009
Accepted:
March 05 2009
Accepted Manuscript online:
March 05 2009
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2009 Biochemical Society
2009
Clin Sci (Lond) (2009) 117 (8): 293–303.
Article history
Received:
October 29 2008
Revision Received:
February 13 2009
Accepted:
March 05 2009
Accepted Manuscript online:
March 05 2009
Citation
Rolf P. Engelberger, Daniel Teta, Hughes Henry, Olivier De Senarclens, Benoît Dischl, Lucas Liaudet, Michel Burnier, Bernard Waeber, François Feihl; Haemodialysis acutely reduces the plasma levels of ADMA without reversing impaired NO-dependent vasodilation. Clin Sci (Lond) 1 October 2009; 117 (8): 293–303. doi: https://doi.org/10.1042/CS20080561
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.