Circulating proteins contribute to the pathogenesis of T2DM (Type 2 diabetes mellitus) in various ways. The aim of the present study was to investigate variations in plasma protein levels in subjects with T2DM and differences in β-cell function, characterized by the EIR (early insulin response), and to compare these protein levels with those observed in individuals with NGT (normal glucose tolerance). Ten subjects with NGT+high EIR, ten with T2DM+high EIR, and ten with T2DM+low EIR were selected from the community-based ULSAM (Uppsala Longitudinal Study of Adult Men) cohort. Plasma protein profiling was performed using SELDI-TOF (surface-enhanced laser-desorption ionization–time-of-flight) MS. In total, nine plasma proteins differed between the three study groups (P<0.05, as determined by ANOVA). The levels of two forms of transthyretin, haemoglobin α-chain and haemoglobin β-chain were decreased in plasma from subjects with T2DM compared with subjects with NGT, irrespective of the EIR of the subjects. Apolipoprotein H was decreased in plasma from individuals with T2DM+high EIR compared with subjects with NGT. Four additional unidentified plasma proteins also varied in different ways between the experimental groups. In conclusion, the proteins detected in the present study may be related to the development of β-cell dysfunction.
Skip Nav Destination
Article navigation
April 2008
-
Cover Image
Cover Image
- PDF Icon PDF LinkTable of Contents
Research Article|
February 29 2008
Plasma proteome changes in subjects with Type 2 diabetes mellitus with a low or high early insulin response
Tea Sundsten;
*Department of Medical Cell Biology, Uppsala University, SE-751 23 Uppsala, Sweden
Correspondence: Tea Sundsten (email Tea.Sundsten@mcb.uu.se).
Search for other works by this author on:
Björn Zethelius;
Björn Zethelius
†Department of Public Health/Geriatrics, Uppsala University, SE-751 23 Uppsala, Sweden
Search for other works by this author on:
Christian Berne;
Christian Berne
‡Department of Medical Sciences, Uppsala University, SE-751 23 Uppsala, Sweden
Search for other works by this author on:
Peter Bergsten
Peter Bergsten
*Department of Medical Cell Biology, Uppsala University, SE-751 23 Uppsala, Sweden
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
September 13 2007
Revision Received:
October 25 2007
Accepted:
October 26 2007
Accepted Manuscript online:
October 26 2007
Online ISSN: 1470-8736
Print ISSN: 0143-5221
© The Authors Journal compilation © 2008 Biochemical Society
2008
Clin Sci (Lond) (2008) 114 (7): 499–507.
Article history
Received:
September 13 2007
Revision Received:
October 25 2007
Accepted:
October 26 2007
Accepted Manuscript online:
October 26 2007
Citation
Tea Sundsten, Björn Zethelius, Christian Berne, Peter Bergsten; Plasma proteome changes in subjects with Type 2 diabetes mellitus with a low or high early insulin response. Clin Sci (Lond) 1 April 2008; 114 (7): 499–507. doi: https://doi.org/10.1042/CS20070323
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.