In the present study, we have analysed the mechanisms of Ca2+ entry and release in platelets obtained from BDL (bile-duct-ligated) rats, 11–13 days and 4 weeks after surgery. Platelets were washed and loaded with fura-2, and [Ca2+]i (cytosolic Ca2+ concentration) was determined in cell suspensions by means of fluorescence spectroscopy. Basal [Ca2+]i was similar in platelets from BDL rats compared with those from their respective controls, both in the absence and presence of extracellular Ca2+. Platelet stimulation with thrombin in the absence and presence of extracellular Ca2+ induced a rapid rise in [Ca2+]i that was of greater magnitude in platelets from BDL rats than in controls. Ca2+ storage was significantly elevated in platelets from BDL rats, as well as the activity of SERCA (sarcoplasmic/endoplasmic-reticulum Ca2+-ATPase). Capacitative Ca2+ entry, as evaluated by inhibition of SERCA with thapsigargin, was also altered in platelets from BDL rats, having lower rates of Ca2+ entry. In conclusion, chronic BDL alters intracellular Ca2+ homoeostasis in platelets, such that an enhanced Ca2+ release is evoked by thrombin, which may be due to an increased amount of Ca2+ stored in the intracellular organelles and secondary to an enhanced activity of SERCA. These alterations are already evident before cirrhosis has completely developed and occurs during the cholestasis phase.
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February 2007
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Research Article|
January 03 2007
Altered calcium signalling in platelets from bile-duct-ligated rats
Noemí M. Atucha;
*Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain
Correspondence: Professor Noemí M. Atucha (email ntma@um.es).
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David Iyú;
David Iyú
*Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain
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Antonia Alcaraz;
Antonia Alcaraz
*Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain
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Vladimir Rosa;
Vladimir Rosa
*Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain
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Concepción Martínez-Prieto;
Concepción Martínez-Prieto
*Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain
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M. Clara Ortiz;
M. Clara Ortiz
*Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain
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Juan Antonio Rosado;
Juan Antonio Rosado
†Department of Physiology, University of Extremadura, Cáceres 10071, Spain
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Joaquín García-Estañ
Joaquín García-Estañ
*Department of Physiology, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain
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Publisher: Portland Press Ltd
Received:
August 18 2006
Accepted:
September 01 2006
Accepted Manuscript online:
September 01 2006
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society
2007
Clin Sci (Lond) (2007) 112 (3): 167–174.
Article history
Received:
August 18 2006
Accepted:
September 01 2006
Accepted Manuscript online:
September 01 2006
Citation
Noemí M. Atucha, David Iyú, Antonia Alcaraz, Vladimir Rosa, Concepción Martínez-Prieto, M. Clara Ortiz, Juan Antonio Rosado, Joaquín García-Estañ; Altered calcium signalling in platelets from bile-duct-ligated rats. Clin Sci (Lond) 1 February 2007; 112 (3): 167–174. doi: https://doi.org/10.1042/CS20060226
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