The aim of the present study was to use proteomics to analyse modifications in the level of expression of different proteins in BVSMCs (bovine vascular smooth muscle cells) incubated in the absence and presence of 17β-oestradiol. By using two-dimensional electrophoresis with a pH range of 4–7, we identified several areas on the gels in which the level of expression of proteins were different between control BVSMCs and cells incubated for 24 h with 17β-oestradiol. Changes in several isoforms of α-enolase, HSP60 (heat-shock protein 60), vimentin and PDI (protein disulphide-isomerase) were observed in BVSMCs. The expression of α-enolase isoform 1 was enhanced after 17β-oestradiol treatment. The expression of HSP60 isoform 3, vimentin isoforms 2 and 3 and caldesmon was reduced by 17β-oestradiol. Finally, the expression of PDI isoforms was reduced by 17β-oestradiol. In summary, 17β-oestradiol modified the expression of isoforms of proteins associated with smooth muscle cell proliferation (α-enolase, vimentin and HSP-60), cell contraction (vimentin and caldesmon) and cell redox modulation (PDI). These findings confirm that 17β-oestradiol may modulate a wide range of signalling pathways in vascular smooth muscle cells.
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Research Article|
October 24 2005
Proteomic approach to identify changes in protein expression modified by 17β-oestradiol in bovine vascular smooth muscle cells
Laura Molero;
Laura Molero
1
1Cardiovascular Research Unit, Cardiovascular Institute, Hospital Clínico San Carlos, C/Profesor Martín Lagos s/n, Madrid 28040, Spain
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Antonio García-Méndez;
Antonio García-Méndez
1
1Cardiovascular Research Unit, Cardiovascular Institute, Hospital Clínico San Carlos, C/Profesor Martín Lagos s/n, Madrid 28040, Spain
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Sergio Alonso-Orgaz;
Sergio Alonso-Orgaz
1Cardiovascular Research Unit, Cardiovascular Institute, Hospital Clínico San Carlos, C/Profesor Martín Lagos s/n, Madrid 28040, Spain
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Carolina Carrasco;
Carolina Carrasco
1Cardiovascular Research Unit, Cardiovascular Institute, Hospital Clínico San Carlos, C/Profesor Martín Lagos s/n, Madrid 28040, Spain
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Carlos Macaya;
Carlos Macaya
1Cardiovascular Research Unit, Cardiovascular Institute, Hospital Clínico San Carlos, C/Profesor Martín Lagos s/n, Madrid 28040, Spain
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Antonio J. López Farré
1Cardiovascular Research Unit, Cardiovascular Institute, Hospital Clínico San Carlos, C/Profesor Martín Lagos s/n, Madrid 28040, Spain
Correspondence: Dr Antonio J. López Farré (email ajlopez.hcsc@salud.madrid.org).
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Publisher: Portland Press Ltd
Received:
February 28 2005
Revision Received:
July 01 2005
Accepted:
July 21 2005
Accepted Manuscript online:
July 21 2005
Online ISSN: 1470-8736
Print ISSN: 0143-5221
The Biochemical Society
2005
Clin Sci (Lond) (2005) 109 (5): 457–463.
Article history
Received:
February 28 2005
Revision Received:
July 01 2005
Accepted:
July 21 2005
Accepted Manuscript online:
July 21 2005
Citation
Laura Molero, Antonio García-Méndez, Sergio Alonso-Orgaz, Carolina Carrasco, Carlos Macaya, Antonio J. López Farré; Proteomic approach to identify changes in protein expression modified by 17β-oestradiol in bovine vascular smooth muscle cells. Clin Sci (Lond) 1 November 2005; 109 (5): 457–463. doi: https://doi.org/10.1042/CS20050082
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