... changes during the Roco G-protein cycle. Our results confirm that the COR domains are a stable dimerization device serving as a scaffold for the Roc domains that, in contrast, are structurally heterogeneous and dynamic entities. Contrary to other GAD proteins, we observed only minor structural alterations...

... target. A serious obstacle to studying GPCR structure/function characteristics is the requirement to extract the receptors from their native environment in the plasma membrane, coupled with the inherent instability of GPCRs in the detergents required for their solubilization. In the present study, we...

... of human Sirt1 and functions of its N- and C-terminal extensions, we recombinantly produced Sirt1 and Sirt1 deletion constructs as well as the AROS (active regulator of Sirt1) protein. We then studied Sirt1 features such as molecular size, secondary structure and stimulation by small molecules and AROS. We...

..., the structure, functional regulation and signalling properties of these integrins are reviewed. How does the α invariant glutamate residue and the β I-like domain MIDAS interaction regulate I domain ligand binding? The I domain can adopt two conformations. The I domain in the closed conformation has low...

... (StAR) structure In steroidogenic cells, cholesterol is converted into pregnenolone by the cytochrome P450 side-chain-cleavage enzyme which is located on the matrix side of the IMM (inner mitochondrial membrane) [ 1 , 2 ]. StAR (steroidogenic acute regulatory protein) (STARD1) regulates...

...+ induced difference FTIR spectra of Ca 2+ -ATPase triggered by photolysis of caged Ca 2+ are consistent with changes in secondary structure and carboxylate groups upon Ca 2+ binding; the changes are reversed during ATP hydrolysis suggesting that a phosphorylated enzyme form of low Ca 2+ affinity...