...Yan Peng; Xin Huang; Tianfang Huang; Feng Du; Xin Cui; Zhuo Tang Herein, Broccoli/ mCherry and an EGFP / mCherry dual-color fluorescent reporting systems have been established to quantify the promoter activity at transcription and translation levels in eukaryotic cells. Based on those systems, four...

...Xin Zhao; Di Cao; Zhangyong Ren; Zhe Liu; Shaocheng Lv; Jiqiao Zhu; Lixin Li; Ren Lang; Qiang He Background: Hypermethylation of gene promoters plays an important role in tumorigenesis. The present study aimed to identify and validate promoter methylation-driven genes (PMDGs) for pancreatic ductal...

...Zaur M. Kachaev; Lyubov A. Lebedeva; Eugene N. Kozlov; Yulii V. Shidlovskii Early stages of transcription from eukaryotic promoters include two principal events: the capping of newly synthesized mRNA and the transition of RNA polymerase II from the preinitiation complex to the productive elongation...

...Ruifen Sun; Jianyu Gong; Ju Li; Zhiguo Ruan; Xiaomi Yang; Yongren Zheng; Lili Qing; Xiaoshan He; Jike Jiang; Yanxia Peng; Haijian Zou Growing evidence has demonstrated that single-nucleotide polymorphisms (SNPs) in the promoter of miRNA may influence individuals’ susceptibility to human diseases...

..., is expressed ubiquitously in various tissues but with higher expression levels in oxidative tissues, such as brain and oxidative muscles. Both the expression level and promoter activity of FoxO6 were found to be enhanced by PGC-1α (peroxisome-proliferator-activated receptor γ co-activator 1α), thus explained...

... is known about the transcriptional regulation and the promoter of this important tumour suppressor. So far the following is the first comprehensive study to describe the regulation of Pdcd4 transcription by ZBP-89 (zinc-finger-binding protein 89), besides characterizing the gene promoter. We identified...

... transcription factors, inflammatory cytokines and factors of cell survival, proliferation and angiogenesis. miR-21 is overexpressed in many tumours, promoting progression and metastasis. In the present study, we examined the potential of curcumin to regulate miR-21, tumour growth, invasion and in vivo...

...Tommaso Beccari; Chiara Balducci; M. Cristina Aisa; M. Agnese Della Fazia; Giuseppe Servillo; Aldo Orlacchio Genomic clones of the human GM2 activator protein have been isolated and analyzed. The 5′ region of the gene demonstrated promoter activity as ascertained by its ability to drive luciferase...

...Ariane van der Straten; Rosette Loriau; Albert Herzog; Alex Bollen Various constructions of human haptoglobin (Hp) cDNA coding either for the complete α 2FS β precursor protein or only for the β subunit have been placed under the control of the λP R promoter in the bacterial expression vector pCQV2...