... progesterone binding. Here, we used small-angle X-ray scattering (SAXS) to investigate the influence of ligand binding and oxidation on apoD oligomerisation and conformation. As a solution-based technique, SAXS is well suited to detect changes in oligomeric state and conformation in response to ligand binding...

... of oligomeric states and indeed, it is known that the oligomerization state of a GPCR can modulate its pharmacology and function. A number of experimental techniques have been devised to study GPCR oligomerization including those based upon traditional biochemistry such as blue-native PAGE (BN-PAGE), co...

... is unable to interact with self or with wt hENT1. Based on these data we propose that glycosylation at N48 is critical for the localization, function and oligomerization of hENT1. 1 To whom correspondence should be addressed (email imogen.coe@ryerson.ca ). 29 2 2016 5 5 2016 1 8...

... expressing controlled combinations of VASP, Mena and EVL, we evaluated the composition of Ena/VASP oligomers and found that VASP forms oligomers without apparent bias with itself, Mena or EVL. However, Mena and EVL showed only weak hetero-oligomerization, suggesting specificity in the association of Ena/VASP...

..., attributable to reduced turnover of hRFC transcripts. hRFC homo-oligomerization was unaffected by increasing LCV. Cell surface hRFC paralleled [ 3 H]methotrexate transport and increased from 0.5 to 2 nM LCV, and then decreased (~2-fold) with increasing LCV up to 20 nM. hRFC was localized to the cell surface...

... and regulation, however, are little understood. It has a unique N-terminal domain and CTD (C-terminal domain) flanking a conserved Sirtuin catalytic core and these extensions are assumed to mediate Sirt1-specific features such as homo-oligomerization and activation by resveratrol. To analyse the architecture...

... ] and stored in sterile PBS. Oligomerization states of purified adiponectin were confirmed using gel filtration chromatography [ 6 ]. Approximately 95% of purified adiponectin consists of 18mers with the balance being hexamers. Hexamers were derived from purified 18mers by adjusting the pH to 4 using glycine...

... movement into the exoplasmic space and the lipid bilayer. 1 email zlcheng2007@berkeley.edu 18 11 2009 6 1 2010 15 1 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 channel opening membrane protein integration oligomerization protein...

... chaperone oligomerization parasite small heat-shock protein (sHsp) stress Giardia lamblia is a flagellated parasite of humans and other vertebrates and is one of the main causes of intestinal diarrhoea worldwide [ 1 ]. Although G. lamblia 's phylogenetic position is still under debate [ 2 , 3...

... fusion viral glycoprotein heptad repeats proteolytic processing glycosylation oligomerization fusion peptides Bioscience Reports, Vol. 20, No. 6, 2000 MINI REVIEW Virus Membrane Fusion Proteins: Biological Machines that Undergo a Metamorphosis Rebecca Ellis Dutch,1 Theodore S. Jardetzky,2...