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Keywords: nuclease
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Simon D.B. Cass, Karina A. Haas, Britta Stoll, Omer S. Alkhnbashi, Kundan Sharma, Henning Urlaub, Rolf Backofen, Anita Marchfelder, Edward L. Bolt
Journal:
Bioscience Reports
Biosci Rep (2015) 35 (3): e00197.
Published: 11 June 2015
... to identify that Cas8 is essential for CRISPR interference. We provide evidence that Cas8 functions as part of archaeal Cascade, an R-loop forming nucleoprotein complex, recognizing protospacer adjacent motifs (PAMs) on invader DNA. An RNA nuclease activity of Cas8 is also described. archaea CRISPR...
Includes: Supplementary data
