... used qRT-PCR to detect the expression levels of miR-92d-3p in the kidneys of patients with DN. Then, after transfecting lentiviruses containing miR-92d-3p into the kidneys of a DN mouse model and HK-2 cell line, we used qRT-PCR to detect the expression levels of miR-92d-3p, C3, HMGB1, TGF-β1, α-SMA, E...

... Figure 6 Repsox represses translocation of Smad3 and Smad4 into nucleus resulting in attenuation of the effect of TGF-β1 on Cx43 ( D ) Representative IF staining by CLSM showing reduced translocation of Smad4 in osteocytes after pretreatment with 50 μM Repsox for 6 h and then treatment...

... neoplasms TGF-β1 Despite significant advances in cancer research, cancer remains a worldwide health problem and mortality due to cancer remains high. Gastric cancer remains the fifth most prevalent cancer worldwide and the third leading cause of global cancer mortality [ 1 ]. There appears...

... © 2020 The Author(s). 2020 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . Connexin 43 Gap junction Osteocyte Smad3 Smad4 TGF-β1 The Editorial...

...) and Smad3 were measured by Western blot. The interaction between miR-203a-3p and SIX1 was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. MiR-203a-3p was down-regulated and SIX1 was up-regulated in asthma serums, respectively. Transforming growth factor-β1 (TGF-β1...

...Jingjing Xie; Bo Li; Bing Yao; Pingchao Zhang; Lixin Wang; Hua Lu; Xuan Song Background: During disc degeneration, inflammatory cytokine tumor necrosis factor (TNF)-α is correlated with nucleus pulposus (NP) cell apoptosis. Transforming growth factor (TGF)-β1 has the potential to regenerate...

...Lili Wu; Guofan Chen; Jia Song Transforming growth factor (TGF)-β1 contributed to angiotensin II (Ang II)-mediated collagen accumulation after myocardial infarction (MI). The present study aimed to investigate the association of genetic variant of TGF-β1 gene with the risk of MI. The present study...

... was transfected into CSCC cells and the effects on cell migration and invasion as well as TGF-β1 expression were investigated by Transwell migration assay, Transwell invasion assay, and Western blot, respectively. We found that expression of NEF in cervical tissues (tumor tissues for CSCC patients) and serum...

... the wound healing on the full-thickness skin wound model. Inflammatory assay results demonstrated that OA-GL12 induced the secretion of tumor necrosis factor (TNF) and transforming growth factor β1 (TGF-β1) on murine macrophage cell line (RAW264.7), which might explain the powerful accelerating capacity...

...Wei Li; Xingyu Wu; Wensheng She Up-regulation of lncRNA POU3F3 has been observed in esophageal squamous cell carcinomas, while its expression pattern and functionality in other human disease is unknown. Our study showed that plasma levels of lncRNA POU3F3 and TGF-β1 (transforming growth factor-β...

.... A significant negative correlation between plasma levels of HAND2-AS1 and TGF-β1 was found in NSCLC patients but not in healthy controls. LncRNA HAND2-AS1 overexpression inhibits, while exogenous TGF-β1 treatment promotes cell migration and invasion ability and cancer cell stemness. Cancer cells with lncRNA...

...Wenjing Liu; Yujia Cui; Jianxun Sun; Linyi Cai; Jing Xie; Xuedong Zhou Connexin 43 (Cx43)-mediated gap junctional intercellular communication (GJIC) has been shown to be important in regulating multiple functions of bone cells. Transforming growth factor-β1 (TGF-β1) exhibited controversial effects...

... subjected to surgical resection and followed-up for 5 years to record cancer recurrence. lncRNA snaR expression vectors were transfected into HPV-negative CSCC cells. Cell migration and invasion ability were evaluated by Transwell migration and invasion assay, respectively. Expression levels of TGF-β1 were...

... of both ovarian carcinoma patients and healthy females was detected by qRT-PCR. Plasma TGF-β1 was detected by ELISA. Correlation between plasma LINK-A and TGF-β1 was analyzed by Pearson correlation analysis. Correlation between plasma LINK-A and patients’ clinicopathological data was analyzed by Chi...

...Su-Zhen Wu; Si-Jun Yang; Hong-Min Chen; Fang-Fang Peng; Hong Yu; Joan C. Krepinsky; Bai-Fang Zhang Little is known about the cross-talk between parathyroid hormone (PTH) related protein (PTHrP) and TGF-β1 in mesangial cells (MCs). Our results showed that PTHrP treatment (≤3 h) induced...

... by transforming growth factor-β1 (TGF-β1); however, the molecular mechanisms remain unknown. In the present study, we tested the efficacy of four selective agonists of PGE 2 receptor, EP 1 A (17-phenyl trinor prostaglandin E 2 ethyl amid), EP 2 A (butaprost), EP 3 A (sulprostone) and EP 4 A (cay10580), on mice...