... BY) . Aristaless-related homeobox Cortex development Mass Spectrometry phosphorylation Post-translational modification of proteins is an essential cellular process that influences the protein localization, function, and degradation. Phosphorylation is one of the most common modifications that can take...

... reaction. The product of this reaction can be selectively detected and quantified with high sensitivity using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform. Further, using this method, we capture and quantify the unique acyl chain composition of PI5P from Drosophila cells and tissues...

... 2019 26 04 2019 © 2019 The Author(s). 2019 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . amino acid metabolism mass spectrometry parasitic...

...-Chao Kidney stone mass spectrometry metabolomics Oxalate crystal Nephrolithiasis is a highly prevalent disease worldwide with rates ranging from 1 to 13% [ 1 ]. The prevalence is progressively rising due to various factors including metabolic dysfunction [ 2 , 3 ] and diet [ 4...

... forms of AKT are present in melanoma cells nuclei. Using mass spectrometry in combination with protein-crosslinking and co-immunoprecipitation, we identified a series of putative protein partners of nuclear AKT, including heterogeneous nuclear ribonucleoprotein (hnRNP), cytoskeleton proteins β-actin, γ...

... 28 10 2018 © 2018 The Author(s). 2018 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . Drosophila lipids membranes mass spectrometry phospholipase...

... Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . calcareous corpuscles host proteins Mass spectrometry metabolically labeled proteins Taenia crassiceps Taenia solium During the last decades, considerable...

... ]. In this context, we concentrated on Mycobacterium tuberculosis , an intracellular pathogen causing tuberculosis, a disease responsible for considerable human mortality worldwide [ 9 ]. enzyme kinetics mass spectrometry molecular dynamic simulations phosphorylation/dephosphorylation site-directed...

... their influence on nerve cell growth. Unusual results compelled us to analyse the composition and purity of both preparations of these proteoglycans (PGs) using both mass spectrometry (MS) and Western blotting, with and without various enzymatic deglycosylations. Commercial ‘decorin’ and ‘biglycan’ were found...

...Monika Kijewska; Karolina Radziszewska; Marta Cal; Mateusz Waliczek; Piotr Stefanowicz; Zbigniew Szewczuk The combination of deuterium–hydrogen exchange (DHX) and mass spectrometry (MS) can be used for studying a high pressure denaturation (HPD) of proteins. Herein we present the results...

...) in 20 mM Tris/HCl (pH 7.4) containing 150 mM NaCl and 10 mM CaCl 2 at 37 °C. carboxypeptidase A cardiovascular disease chymase mass spectrometry mast cell truncated apolipoprotein A-I MCs (mast cells) distribute to mucosal surfaces and skin throughout the body and play an important...

...@liv.ac.uk ). 22 6 2010 1 9 2010 14 9 2010 14 9 2010 © The Authors Journal compilation © 2011 Biochemical Society 2011 elemental analysis fetal calf serum (FCS) flow cytometry human umbilical-vein endothelial cell (HUVEC) in vitro mass spectrometry...

...Ida Chiara Guerrera; Oliver Kleiner Mass spectrometry has arguably become the core technology in proteomics. The application of mass spectrometry based techniques for the qualitative and quantitative analysis of global proteome samples derived from complex mixtures has had a big impact...

... Springer 2005 Functional proteomics mass spectrometry protein–protein interactions affinity-based strategies Abbreviations 1D-gel, monodimensional gel electrophoresis; 2D-gel, two-dimensional gel electrophoresis; AldA-NRE, Aldolase A negative regulatory element; ES-LC-MS/MS...

... ribofolate peptide folate folinic acid thymic extract sheep thymus thymocytes mass spectrometry Bioscience Reports, Vol. I1, No. 2, 1991 Identification of a Mammalian Growth Factor as a Ribofolate Peptide Ul f Ernst r6m 1 Received April 5, 1991 Thymocyte growth peptide (TGP) promotes DNA synthesis...