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Keywords: CD
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Biosci Rep (2010) 30 (5): 359–364.
Published: 03 June 2010
... at 10°C, using an SLM Aminco spectrofluorimeter (Spectronic Instruments, Rochester, NY, U.S.A.) with cell stirring. Far-UV CD spectra were recorded typically at 0.2 nm resolution on a Jasco J-815 spectropolarimeter (Jasco, Tokyo, Japan) fitted with a cell holder thermostatically controlled by a Peltier...
Biosci Rep (2010) 30 (5): 341–349.
Published: 21 April 2010
... corresponding to a molecular mass of 23.7 kDa, whereas ESI-MS (electrospray ionization MS) gave the molecular masses of the subunit as 24645 Da. Analysis of the CD spectrum of the protein indicated that the secondary structure of the lectin consists of 9.7% α-helix, 35.8% β-sheet, 22.5% β-turn and 32.3...
Includes: Supplementary data
Biosci Rep (2010) 30 (2): 111–117.
Published: 23 November 2009
... range. Far-CD spectroscopic analysis revealed an increase in the helicity of the enzyme at acidic pH in the presence of tannic acid but no change at alkaline pH. Fluorescence spectra revealed a red shift in λ max of the enzyme, suggesting that tryptophan residues come to a more hydrophilic environment...
Biosci Rep (1999) 19 (6): 589–600.
Published: 01 December 1999
... molecules in its structure, which possibly stabilize the enzyme and might be exchanged in ethanol solutions. © 1999 Plenum Publishing Corporation 1999 Lacase mediator ethanol solvents water chemical activity CD Bioscience Reports, Vol. 19, No. 6, 1999 Effect of Ethanol on Enzymatic Activity...