... at 10°C, using an SLM Aminco spectrofluorimeter (Spectronic Instruments, Rochester, NY, U.S.A.) with cell stirring. Far-UV CD spectra were recorded typically at 0.2 nm resolution on a Jasco J-815 spectropolarimeter (Jasco, Tokyo, Japan) fitted with a cell holder thermostatically controlled by a Peltier...

... corresponding to a molecular mass of 23.7 kDa, whereas ESI-MS (electrospray ionization MS) gave the molecular masses of the subunit as 24645 Da. Analysis of the CD spectrum of the protein indicated that the secondary structure of the lectin consists of 9.7% α-helix, 35.8% β-sheet, 22.5% β-turn and 32.3...

... range. Far-CD spectroscopic analysis revealed an increase in the helicity of the enzyme at acidic pH in the presence of tannic acid but no change at alkaline pH. Fluorescence spectra revealed a red shift in λ max of the enzyme, suggesting that tryptophan residues come to a more hydrophilic environment...

...Ejaz Ahmad; Shah Kamranur Rahman; Javed Masood Khan; Ankita Varshney; Rizwan Hasan Khan This is the first report of its kind that well demonstrates that a lectin from Phytolacca americana [Pa-2 ( P. americana lectin-2)] can also be intrinsically unordered, based on the results obtained by CD...

... ) in the cholesterol-binding site is also proposed to be critical to the binding process. Interestingly, some of the documented clinical mutations occur at this salt bridge (E169G, E169K and R188C) and in the C-terminal α-helix 4 (L275P). In the present study, using rationalized mutagenesis, activity assays, CD...

... molecules in its structure, which possibly stabilize the enzyme and might be exchanged in ethanol solutions. © 1999 Plenum Publishing Corporation 1999 Lacase mediator ethanol solvents water chemical activity CD Bioscience Reports, Vol. 19, No. 6, 1999 Effect of Ethanol on Enzymatic Activity...