A novel antioxidant polysaccharide–peptide complex LB-1b from the fruiting bodies of the edible abalone mushroom (Pleurotus abalonus) was purified and identified. The structural characteristic of LB-1b was identified by FTIR (Fourier-transform IR), 13C NMR and 1H NMR spectroscopy. LB-1b is a polysaccharide–peptide complex that contains glucose, rhamnose, glucuronic acid and galactose in the molar ratio of 22.4:1:1.7:1.6 and the N-terminal sequence of its peptide moiety has also been determined. The N-terminal amino acid sequence of LB-1b, IPKERKEFQQAQHLK, showed some resemblance to antioxidant enzymes. LB-1b exhibited high antioxidant activity in erythrocyte haemolysis in vitro and the anti-proliferative activity towards hepatoma HepG2 cells and breast cancer MCF7 cells with an IC50 of 24 and 14 μM respectively. LB-1b also demonstrated hypoglycaemic activity in drug-induced diabetic mice and anti-HIV-1 RT (reverse transcriptase) with an IC50 value of 12.5 μM.
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Research Article|
December 22 2011
Isolation and identification of a novel polysaccharide–peptide complex with antioxidant, anti-proliferative and hypoglycaemic activities from the abalone mushroom
Ning Li;
Ning Li
*Department of Microbiology, College of Life Science, Nankai University, Tianjin, People's Republic of China
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Le Li;
Le Li
†Research Center of Fishery Production Standard and Quality Chinese Academy of Fishery Sciences, Beijing, People's Republic of China
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Jin Cen Fang;
Jin Cen Fang
†Research Center of Fishery Production Standard and Quality Chinese Academy of Fishery Sciences, Beijing, People's Republic of China
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Jack Ho Wong;
Jack Ho Wong
‡School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, People's Republic of China
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Tzi Bun Ng;
Tzi Bun Ng
1
‡School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, People's Republic of China
1Correspondence may be addressed to either of these authors (email b021770@mailserv.cuhk.edu.hk or liufang312@nankai.edu.cn).
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Yun Jiang;
Yun Jiang
*Department of Microbiology, College of Life Science, Nankai University, Tianjin, People's Republic of China
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Chang Rong Wang;
Chang Rong Wang
*Department of Microbiology, College of Life Science, Nankai University, Tianjin, People's Republic of China
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Ni Ye Zhang;
Ni Ye Zhang
*Department of Microbiology, College of Life Science, Nankai University, Tianjin, People's Republic of China
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Ting Yi Wen;
Ting Yi Wen
§Key Laboratory of Systems Microbial Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China
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Li Yuan Qu;
Li Yuan Qu
*Department of Microbiology, College of Life Science, Nankai University, Tianjin, People's Republic of China
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Peng Yun Lv;
Peng Yun Lv
*Department of Microbiology, College of Life Science, Nankai University, Tianjin, People's Republic of China
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Ruili Zhao;
Ruili Zhao
*Department of Microbiology, College of Life Science, Nankai University, Tianjin, People's Republic of China
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Bin Shi;
Bin Shi
*Department of Microbiology, College of Life Science, Nankai University, Tianjin, People's Republic of China
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Yin Ping Wang;
Yin Ping Wang
*Department of Microbiology, College of Life Science, Nankai University, Tianjin, People's Republic of China
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Xiao Ying Wang;
Xiao Ying Wang
*Department of Microbiology, College of Life Science, Nankai University, Tianjin, People's Republic of China
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Fang Liu
Fang Liu
1
*Department of Microbiology, College of Life Science, Nankai University, Tianjin, People's Republic of China
1Correspondence may be addressed to either of these authors (email b021770@mailserv.cuhk.edu.hk or liufang312@nankai.edu.cn).
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Publisher: Portland Press Ltd
Received:
February 18 2011
Revision Received:
July 04 2011
Accepted:
August 03 2011
Accepted Manuscript online:
August 03 2011
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2012 Biochemical Society
2012
Biosci Rep (2012) 32 (3): 221–228.
Article history
Received:
February 18 2011
Revision Received:
July 04 2011
Accepted:
August 03 2011
Accepted Manuscript online:
August 03 2011
Citation
Ning Li, Le Li, Jin Cen Fang, Jack Ho Wong, Tzi Bun Ng, Yun Jiang, Chang Rong Wang, Ni Ye Zhang, Ting Yi Wen, Li Yuan Qu, Peng Yun Lv, Ruili Zhao, Bin Shi, Yin Ping Wang, Xiao Ying Wang, Fang Liu; Isolation and identification of a novel polysaccharide–peptide complex with antioxidant, anti-proliferative and hypoglycaemic activities from the abalone mushroom. Biosci Rep 1 June 2012; 32 (3): 221–228. doi: https://doi.org/10.1042/BSR20110012
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