Membrane fusion is one of the most important cellular processes by which two initially distinct lipid bilayers merge their hydrophobic cores, resulting in one interconnected structure. Proteins, called SNARE (soluble N-ethylmaleimide-sensitive factor-attachment protein receptor), play a central role in the fusion process that is also regulated by several accessory proteins. In order to study the SNARE-mediated membrane fusion, the in vitro protein reconstitution assay involving ensemble FRET (fluorescence resonance energy transfer) has been used over a decade. In this mini-review, we describe several single-molecule-based FRET approaches that have been applied to this field to overcome the shortage of the bulk assay in terms of protein and fusion dynamics.
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Review Article|
September 15 2011
Single-molecule FRET study of SNARE-mediated membrane fusion
Jiajie Diao;
*Department of Physics and Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801, U.S.A.
2Correspondence may be addressed to either of these authors (email yuji@illinois.edu or diaojj@stanford.edu).
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Yuji Ishitsuka;
Yuji Ishitsuka
2
*Department of Physics and Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801, U.S.A.
†Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, Urbana, IL 61801, U.S.A.
2Correspondence may be addressed to either of these authors (email yuji@illinois.edu or diaojj@stanford.edu).
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Woo-Ri Bae
Woo-Ri Bae
‡Department of Physics, KAIST, Daejeon 305-701, Republic of Korea
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Publisher: Portland Press Ltd
Received:
February 07 2011
Accepted:
February 23 2011
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biosci Rep (2011) 31 (6): 457–463.
Article history
Received:
February 07 2011
Accepted:
February 23 2011
Citation
Jiajie Diao, Yuji Ishitsuka, Woo-Ri Bae; Single-molecule FRET study of SNARE-mediated membrane fusion. Biosci Rep 1 December 2011; 31 (6): 457–463. doi: https://doi.org/10.1042/BSR20110011
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