In the present study, two antioxidant micromolecular components (L2f-2 and L2f-3) and an antioxidant macromolecular component LB2 were extracted from lotus (Nelumbo nucifera Gaertn.) rhizomes. MS, FTIR (Fourier-transform IR) spectroscopy and NMR were used to identify these compounds. L2f-2 was (+/−)-gallocatechin, L2f-3 was (−)-catechin and LB2 was a polysaccharide–protein complex with a molecular mass of 18.8 kDa. LB2 was identified as a polysaccharide sulfate containing α/β-pyranose and α-furanose according to its FTIR spectrogram. It was composed of mannose, rhamnose, glucose, galactose and xylose with a molar ratio 2:8:7:8:1. The antioxidant components L2f-2, L2f-3 and LB2 strongly inhibited HIV-1 RT (reverse transcriptase) and IN (integrase). LB2 inhibited RT with an IC50 value of 33.7 μM. It also exhibited the highest HIV-1 3′-processing inhibitory activity with an IC50 value of 5.28 μM. Both L2f-2 and L2f-3 up-regulated the expression of IL-2 (interleukin-2) and down-regulated IL-10, while LB2 exhibited positive regulation on IL-2, IL-4 and IL-10. Moreover, L2f-3 and LB2 might inhibit HIV-1 directly by down-regulating TNFα (tumour necrosis factor α). These natural antioxidant components with antiviral and immunoregulatory activities could be potentially important for anti HIV-1 drug development and application to HIV-1 therapy.
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Research Article|
August 05 2011
Immunoregulatory and anti-HIV-1 enzyme activities of antioxidant components from lotus (Nelumbo nucifera Gaertn.) rhizome
Yun Jiang;
Yun Jiang
*Center for AIDS Research, Nankai University, Tianjin, People's Republic of China
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Tzi Bun Ng;
Tzi Bun Ng
1
†School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, People's Republic of China
1Correspondence may be addressed to either of these authors (email b021770@mailserv.cuhk.edu.hk or liufang312@nankai.edu.cn).
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Zhaokun Liu;
Zhaokun Liu
*Center for AIDS Research, Nankai University, Tianjin, People's Republic of China
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Changrong Wang;
Changrong Wang
*Center for AIDS Research, Nankai University, Tianjin, People's Republic of China
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Ning Li;
Ning Li
*Center for AIDS Research, Nankai University, Tianjin, People's Republic of China
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Wentao Qiao;
Wentao Qiao
*Center for AIDS Research, Nankai University, Tianjin, People's Republic of China
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Fang Liua
Fang Liua
1
‡Department of Microbiology, Nankai University, Tianjin, People's Republic of China
1Correspondence may be addressed to either of these authors (email b021770@mailserv.cuhk.edu.hk or liufang312@nankai.edu.cn).
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Publisher: Portland Press Ltd
Received:
July 13 2010
Revision Received:
November 08 2010
Accepted:
November 30 2010
Accepted Manuscript online:
November 30 2010
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biosci Rep (2011) 31 (5): 381–390.
Article history
Received:
July 13 2010
Revision Received:
November 08 2010
Accepted:
November 30 2010
Accepted Manuscript online:
November 30 2010
Citation
Yun Jiang, Tzi Bun Ng, Zhaokun Liu, Changrong Wang, Ning Li, Wentao Qiao, Fang Liua; Immunoregulatory and anti-HIV-1 enzyme activities of antioxidant components from lotus (Nelumbo nucifera Gaertn.) rhizome. Biosci Rep 1 October 2011; 31 (5): 381–390. doi: https://doi.org/10.1042/BSR20100062
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