A cell line is an important experimental platform for biological sciences as it can basically reflect the biology of its original organism. In this study, we firstly characterized the proteome of cultured BmN cells, derived from Bombyx mori. Total 1478 proteins were identified with two or more peptides by using 1D (one-dimensional) SDS/PAGE and LTQ-Orbitrap. According to the gene ontology annotation, these proteins presented diverse pI values and molecular masses, involved in various molecular functions, including catalytic activity, binding, molecular transducer activity, motor activity, transcription regulator activity, enzyme regulator activity and antioxidant activity. Some proteins related to virus infection were also identified. These results provided us with useful information to understand the molecular mechanism of B. mori as well as antiviral immunity.
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Research Article|
March 03 2010
Proteomics identification and annotation of proteins of a cell line of Bombyx mori, BmN cells
Hui-peng Yao;
Hui-peng Yao
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
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Lin Chen;
Lin Chen
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
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Xingwei Xiang;
Xingwei Xiang
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
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Ai-qin Guo;
Ai-qin Guo
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
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Xing-meng Lu;
Xing-meng Lu
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
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Xiao-feng Wu
Xiao-feng Wu
1
1College of Animal Science, Zhejiang University, Hangzhou 310029, People's Republic of China
1To whom any correspondence should be addressed (email wuxiaofeng@zju.edu.cn).
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Publisher: Portland Press Ltd
Received:
April 03 2009
Revision Received:
May 29 2009
Accepted:
June 04 2009
Accepted Manuscript online:
June 04 2009
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2010 Biochemical Society
2010
Biosci Rep (2010) 30 (3): 209–215.
Article history
Received:
April 03 2009
Revision Received:
May 29 2009
Accepted:
June 04 2009
Accepted Manuscript online:
June 04 2009
Citation
Hui-peng Yao, Lin Chen, Xingwei Xiang, Ai-qin Guo, Xing-meng Lu, Xiao-feng Wu; Proteomics identification and annotation of proteins of a cell line of Bombyx mori, BmN cells. Biosci Rep 1 June 2010; 30 (3): 209–215. doi: https://doi.org/10.1042/BSR20090045
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