The thermo-responsivity of PNIPAAm [poly(N-isopropylcarylamide)]-grafted PDMS [poly(dimethylsiloxane)] surface is a property that could be feasibly used for detaching cells adhered on the surface. We used benzophenone-initiated photopolymerization to graft PNIPAAm on PDMS substrates to construct the PNIPAAm-grafted PDMS surface and this PDMS surface was highly thermo-responsive. hMSCs (human mesenchymal stem cells) were used to analyse the proliferation and multi-differentiation of stem cells on the PNIPAAm-grafted PDMS surface. The results showed that hMSCs could adhere on the PNIPAAm-grafted PDMS surface at 37°C and form cell colonies, and then become fibroblastic. The proliferation potential of hMSCs on the PNIPAAm-grafted PDMS surface was not significantly different from that on a plate surface coated with gelatin. However, as it proved easier to detach cells from the surface, by changing temperature, a higher viability of detached cells could be obtained with the PNIPAAm-grafted PDMS surface, using a temperature shift, compared with a gelatin-coated surface, where cells are detached by treatment with trypsin. hMSCs on the PNIPAAm-grafted PDMS surface were induced into osteoblasts, adipocytes and neurocytes under osteogenic medium, adipogenic medium and neurogenic medium respectively. The PNIPAAm-grafted PDMS surface was favourable for osteogenesis of hMSCs, although the potentials of adipogenesis and neurogenesis of hMSCs on the PNIPAAm-grafted PDMS surface were similar to those on the plate surface coated with gelatin. The above results demonstrate that the PNIPAAm-grafted PDMS surface not only kept the potentials of proliferation and multi-differentiation of hMSCs, but also increased the viability of hMSCs.
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Research Article|
December 15 2009
Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm
Dongyan Shi;
Dongyan Shi
1
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
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Dan Ma;
Dan Ma
1
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
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Feiqing Dong;
Feiqing Dong
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
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Chen Zong;
Chen Zong
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
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Liyue Liu;
Liyue Liu
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
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Dan Shen;
Dan Shen
†Laboratory of Bone Marrow, The First Hospital, Zhejiang University, Hangzhou, Zhejiang 310006, China
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Wenji Yuan;
Wenji Yuan
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
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Xiangmin Tong;
Xiangmin Tong
‡The Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
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Hengwu Chen;
Hengwu Chen
‡The Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
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Jinfu Wang
Jinfu Wang
2
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
1To whom correspondence should be addressed (email wjfu@zju.edu.cn).
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Publisher: Portland Press Ltd
Received:
February 24 2009
Revision Received:
April 28 2009
Accepted:
May 15 2009
Accepted Manuscript online:
May 15 2009
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2010 Biochemical Society
2010
Biosci Rep (2010) 30 (3): 149–158.
Article history
Received:
February 24 2009
Revision Received:
April 28 2009
Accepted:
May 15 2009
Accepted Manuscript online:
May 15 2009
Connected Content
Citation
Dongyan Shi, Dan Ma, Feiqing Dong, Chen Zong, Liyue Liu, Dan Shen, Wenji Yuan, Xiangmin Tong, Hengwu Chen, Jinfu Wang; Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm. Biosci Rep 1 June 2010; 30 (3): 149–158. doi: https://doi.org/10.1042/BSR20090026
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