This is the first report of its kind that well demonstrates that a lectin from Phytolacca americana [Pa-2 (P. americana lectin-2)] can also be intrinsically unordered, based on the results obtained by CD, tryptophan fluorescence, ANS (8-anilinonaphthalene-1-sulfonic acid) binding, acrylamide quenching, DLS (dynamic light scattering) and its amino acid composition database analyses. Pa-2 is an acidic monomeric lectin and acquires random coil conformation at neutral pH without any regular secondary structure. As confirmed by different spectroscopic techniques, on lowering the pH, some secondary structures, predominantly α-helices, are detected by far-UV CD that adopt a marginally stable partially folded collapsed conformation possessing the characteristics of a premolten globule state. It is in accordance with coil–helix transition that is commonly observed when these intrinsically unordered proteins interact with their partner molecules in vivo.
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Research Article|
November 23 2009
Phytolacca americana lectin (Pa-2; pokeweed mitogen): an intrinsically unordered protein and its conversion into partial order at low pH
Ejaz Ahmad;
Ejaz Ahmad
1Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India
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Shah Kamranur Rahman;
Shah Kamranur Rahman
1Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India
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Javed Masood Khan;
Javed Masood Khan
1Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India
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Ankita Varshney;
Ankita Varshney
1Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India
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Rizwan Hasan Khan
Rizwan Hasan Khan
1
1Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India
1To whom any correspondence should be addressed (email rizwanhkhan@hotmail.com and rizwanhkhan1@yahoo.com).
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Publisher: Portland Press Ltd
Received:
March 10 2009
Revision Received:
April 15 2009
Accepted:
May 13 2009
Accepted Manuscript online:
May 13 2009
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2010 Biochemical Society
2010
Biosci Rep (2010) 30 (2): 125–134.
Article history
Received:
March 10 2009
Revision Received:
April 15 2009
Accepted:
May 13 2009
Accepted Manuscript online:
May 13 2009
Citation
Ejaz Ahmad, Shah Kamranur Rahman, Javed Masood Khan, Ankita Varshney, Rizwan Hasan Khan; Phytolacca americana lectin (Pa-2; pokeweed mitogen): an intrinsically unordered protein and its conversion into partial order at low pH. Biosci Rep 1 April 2010; 30 (2): 125–134. doi: https://doi.org/10.1042/BSR20090035
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