LAB (lithospermic acid B) is a dimer of RA (rosmarinic acid) and has been suggested to be derived from RA, but the detailed biosynthesis process has not yet been identified. The accumulation of RA has been intensively investigated in the plant species of Boraginaceae and Lamiaceae. In the present study, we report that silver ions (Ag+; 15 μM), an abiotic elicitor, did not stimulate RA accumulation but dramatically enhanced LAB from approx. 5.4% to 18.8% of dry weight in Salvia miltiorrhiza hairy root cultures, and the rise in LAB was found to be coincident with the decline of RA content at each time point after treatment. Meanwhile, a profiling analysis of genes and metabolites (intermediates) involved in the RA synthesis pathway was performed; the result indicated that several gene transcripts and metabolite accumulations show temporal changes in abundance consistent with LAB production. Thus a potential (putative) biosynthetic route from RA to LAB was presumed, which was suggested to be significantly activated by Ag+ in S. miltiorrhiza hairy root cultures. Further intermediate monitoring and compound feeding experiments were performed to rank the strength of this hypothesis. Our study, for the first time, provides evidence that RA is a precursor leading to LAB synthesis.
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Research Article|
October 06 2009
Lithospermic acid B is more responsive to silver ions (Ag+) than rosmarinic acid in Salvia miltiorrhiza hairy root cultures
Ying Xiao;
Ying Xiao
*Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, People's Republic of China
†Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China
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Shouhong Gao;
Shouhong Gao
†Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China
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Peng Di;
Peng Di
†Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China
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Junfeng Chen;
Junfeng Chen
†Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China
‡Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, People's Republic of China
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Wansheng Chen;
Wansheng Chen
1
†Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China
‡Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, People's Republic of China
1Correspondence may be addressed to either of these authors (email smmusubmit@gmail.com or starzhanglei@gmail.com).
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Lei Zhang
Lei Zhang
1
*Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, People's Republic of China
1Correspondence may be addressed to either of these authors (email smmusubmit@gmail.com or starzhanglei@gmail.com).
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Publisher: Portland Press Ltd
Received:
September 05 2008
Revision Received:
February 03 2009
Accepted:
February 11 2009
Accepted Manuscript online:
February 11 2009
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2010 Biochemical Society
2010
Biosci Rep (2010) 30 (1): 33–40.
Article history
Received:
September 05 2008
Revision Received:
February 03 2009
Accepted:
February 11 2009
Accepted Manuscript online:
February 11 2009
Citation
Ying Xiao, Shouhong Gao, Peng Di, Junfeng Chen, Wansheng Chen, Lei Zhang; Lithospermic acid B is more responsive to silver ions (Ag+) than rosmarinic acid in Salvia miltiorrhiza hairy root cultures. Biosci Rep 1 February 2010; 30 (1): 33–40. doi: https://doi.org/10.1042/BSR20080124
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