Mitochondrial AOX (alternative oxidase) is the terminal oxidase of the CN (cyanide)-resistant alternative respiratory pathway in plants. To investigate the role of the tobacco AOX gene (NtAOX1a) (where Nt is Nicotiana tabacum) under deleterious conditions which could induce ROS (reactive oxygen species) accumulation, we generated and characterized a number of independent transgenic tobacco (N. tabacum) lines with altered NtAOX1a gene expression and AP (alternative pathway) capacity. AOX efficiently inhibited the production of low-temperature-induced H2O2 and might be a major enzyme for scavenging H2O2 at low temperature. Furthermore, NtAOX1a may act as a regulator of KCN-induced resistance to TMV (tobacco mosaic virus) through the regulation of H2O2. Notably, a moderate accumulation of H2O2 under the control of NtAOX1a was crucial in viral resistance. Analysis of seed germination indicated an important role for NtAOX1a in germination under H2O2-induced oxidative stress when the CP (cytochrome pathway) was inhibited. These results demonstrate that NtAOX1a is necessary for plants to survive low temperature, pathogen attack and oxidative stress by scavenging ROS under these adverse conditions when the CP is restricted.
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Research Article|
July 22 2009
Functional analysis reveals effects of tobacco alternative oxidase gene (NtAOX1a) on regulation of defence responses against abiotic and biotic stresses
Yi Zhang;
Yi Zhang
1
1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China
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Dongmei Xi;
Dongmei Xi
1
1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China
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Jian Wang;
Jian Wang
1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China
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Dongfang Zhu;
Dongfang Zhu
1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China
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Xingqi Guo
Xingqi Guo
2
1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China
2To whom correspondence should be addressed (email xqguo@sdau.edu.cn).
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Publisher: Portland Press Ltd
Received:
September 16 2008
Revision Received:
December 04 2008
Accepted:
January 06 2009
Accepted Manuscript online:
January 06 2009
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biosci Rep (2009) 29 (6): 375–383.
Article history
Received:
September 16 2008
Revision Received:
December 04 2008
Accepted:
January 06 2009
Accepted Manuscript online:
January 06 2009
Citation
Yi Zhang, Dongmei Xi, Jian Wang, Dongfang Zhu, Xingqi Guo; Functional analysis reveals effects of tobacco alternative oxidase gene (NtAOX1a) on regulation of defence responses against abiotic and biotic stresses. Biosci Rep 1 December 2009; 29 (6): 375–383. doi: https://doi.org/10.1042/BSR20080133
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